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JCM 674 . PYG MEDIUM (F)

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J674 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
S I solution 6.0 g/L 75.0 ml - -
S II solution 75.0 ml - -
Trypticase peptone 10.0 g 9.96 -
Yeast extract 5.0 g 4.98 -
Glucose 10.0 g 9.96 55.285
Sodium resazurin
(w/v, 0.1%) 		1.0 ml - -
Na2CO3
(w/v, 8%) 		2.5 ml - -
L-Cysteine HCl x H2O 0.3 g 0.299 -
Distilled water 850.0 ml - -
1 Adjust pH to 7.3.

S I solution 6.0 g/L #

100
Solution occurs in 1 other media (first defined in Medium J674).
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.6 g 6 34.448
Distilled water 100.0 ml - -

S II solution #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.60 g 6 44.09
(NH4)2SO4 1.20 g 12 90.813
NaCl 1.20 g 12 205.339
MgSO4 x 7 H2O 0.12 g 1.2 4.869
CaCl2 x 2 H2O 0.12 g 1.2 8.162
Distilled water 100.00 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.3
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Associated strains

All strains for this medium: 15

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Glucose 9.96 55.285
Trypticase peptone 9.96 -
Yeast extract 4.98 -
NaCl 0.896 15.339
(NH4)2SO4 0.896 6.784
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Cultivation metadata from 15 strains BacDive

pH 6 - 8 8 strains
pH 4 - 6 4 strains
pH 8 - 10 3 strains
pH 2 - 4 1 strain
phyla 2 15 strains
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