J679
679 .
DEHALOGENIMONAS MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J679 #
ml
Equipment needed: Filter
Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 1.00 | g | 0.994 | 17.01 | |
| 1 To complete the medium, adjust pH to 7.0 with sterile HCl solution, dispense into culture vessels leaving 40 to 50% volume of gas phase (e.g., 30 ml of the medium in a 50 ml serum bottle) and seal with butyl rubber stoppers. Replace the gas phase with a N2-CO2-H2 (8:1:1, v/v) gas mixture. Finally add the following filter-sterilized chlorosolvent (per liter): | |||||
| MgCl2 x 6 H2O | 0.40 | g | 0.398 | 1.956 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.099 | 0.676 | |
| NH4Cl | 0.25 | g | 0.249 | 4.646 | |
| KH2PO4 | 0.20 | g | 0.199 | 1.461 | |
| KCl | 0.50 | g | 0.497 | 6.667 | |
| Sodium acetate | 0.41 | g | 0.408 | 4.968 | |
| L-Cysteine HCl x H2O | 0.25 | g | 0.249 | - | |
| Resazurin | 1.00 | mg | 9.9e-4 | 0.004 | |
| Distilled water | 910.00 | ml | - | - | |
| 2 Mix components and autoclave under a N2 atmosphere. After cooling, anaerobically add the following sterile solutions (autoclaved or *filter-sterilized) from anaerobic stocks: | |||||
|
NaHCO3
(*, 8%) |
30.00 | ml | - | - | |
| Trace element solution | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
|
Vitamin solution (see Medium No. 403)
(*) |
10.00 | ml | - | - | |
|
Thiamine solution (see Medium No. 403)
(*) |
10.00 | ml | - | - | |
|
Vitamin B12 solution (see Medium No. 403)
(*) |
10.00 | ml | - | - | |
|
Ampicillin
(*, 0.1 g/ml) |
10.00 | ml | - | - | |
|
Vancomycin x HCl
(*, 10 mg/ml) |
10.00 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
8.00 | ml | - | - | |
|
1,2-Dichloropropane
(10 mg/ml in methanol) |
6.00 | ml | - | - | |
Solution occurs in 38
other media (first defined in Medium J439).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
|
HCl
(25%) |
12.5 | ml | - | - |
| FeSO4 x 7 H2O | 2.1 | g | 2.1 | 7.554 |
| H3BO3 | 30.0 | mg | 0.03 | 0.485 |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | - |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 |
| ZnSO4 x 7 H2O | 144.0 | mg | 0.144 | 0.501 |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 |
| Distilled water | 987.0 | ml | - | - |
Solution occurs in 71
other media (first defined in Medium J431).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaOH | 0.4 | g | 0.4 | 10.001 |
| Na2SeO3 x 5 H2O | 6.0 | mg | 0.006 | 0.023 |
| Na2WO4 x 2 H2O | 8.0 | mg | 0.008 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Defined medium |
| Final pH: | 7.0 |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter |
| Edit in Medium builder | |