Name: FRR MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Main sol. J683 #

Solution A #

1002

Equipment needed: Autoclave Hungate tubes Anaerobic workbench

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NH₄Cl	1.000	g	0.998	18.658
NaNO₃	2.550	g	2.545	29.942
KH₂PO₄	0.080	g	0.08	0.587
MgCl₂ x 6 H₂O	4.500	g	4.491	22.09
CaCl₂ x 2 H₂O	0.375	g	0.374	2.546
NaCl	17.000	g	16.966	290.316
FeCl2 solution	1.000	ml	-	-
Trace element solution	1.000	ml	-	-
NaHCO₃	3.600	g	3.593	42.768
Distilled water	1000.000	ml	-	-
1 To complete the medium, anaerobically and aseptically add the following solutions (autoclaved under a N₂ atmosphere) to the medium (per 10 ml):
Yeast extract (10%)	0.100	ml	-	-
Sodium acetate (1 M)	0.100	ml	-	-
2 Mix components and adjust pH to 7.0. Dispense the solution into culture vessels (e.g., 10 ml in the Hungate tubes) under a N₂-CO₂ (4:1, v/v) gas stream, seal with butyl rubber stoppers and autoclave.
3 Comment: Sodium nitrate can be substituted by ferric citrate (20 mM).

1000

Solution occurs in 87 other media (first defined in Medium J187).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
HCl (7.7 M, 25%)	10.0	ml	2.5	68.568
FeCl₂ x 4 H₂O	1.5	g	1.5	7.545
Distilled water	990.0	ml	-	-

1000

Solution occurs in 89 other media (first defined in Medium J187).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
ZnCl₂	70	mg	0.07	0.514
MnCl₂ x 4 H₂O	100	mg	0.1	0.505
H₃BO₃	6	mg	0.006	0.097
CoCl₂ x 6 H₂O	190	mg	0.19	1.463
CuCl₂ x 2 H₂O	2	mg	0.002	0.012
NiCl₂ x 6 H₂O	24	mg	0.024	0.185
Na₂MoO₄ x 2 H₂O	36	mg	0.036	0.149
Distilled water	1000	ml	-	-

Last modified:	22.02.22
Source:	JCM
Taxonomic range:	Bacteria
Medium type:	Complex medium
Final pH:	7.0
Equipment needed:	Anaerobic workbench, Autoclave, Hungate tubes

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