Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 0.75 | g | 0.73 | 5.366 | |
K2HPO4 | 1.50 | g | 1.461 | 8.386 | |
NaCl | 0.90 | g | 0.876 | 14.996 | |
MgCl2 x 6 H2O | 0.20 | g | 0.195 | 0.958 | |
NH4Cl | 0.90 | g | 0.876 | 16.383 | |
Trace mineral solution | 9.00 | ml | - | - | |
Trace vitamins | 10.00 | ml | - | - | |
FeSO4 x 7 H2O
(10%) |
5.00 | �l | - | - | |
Tryptone
(BD-Difco) |
2.00 | g | 1.947 | 27.398 | |
Yeast extract | 2.00 | g | 1.947 | - | |
Glucose | 2.00 | g | 1.947 | 10.809 | |
Resazurin | 1.00 | mg | 9.7e-4 | 0.004 | |
Distilled water | 1000.00 | ml | - | - | |
1 Mix components and adjust pH to 7.5. Bring to a boil, then cool down and distribute into culture vessels under a N2 gas stream. Seal with butyl rubber stoppers and autoclave. Prior to inoculation, add the following solution (autoclaved under a N2 atmosphere) into the medium (per liter): | |||||
Na2S x 9 H2O
(5%) |
8.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 12.50 | g | 12.5 | 65.397 | |
FeCl2 x 4 H2O | 0.20 | g | 0.2 | 1.006 | |
MnCl2 x 4 H2O | 0.10 | g | 0.1 | - | |
CoCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
ZnCl2 | 0.10 | g | 0.1 | 0.734 | |
CuCl2 | 0.20 | g | 0.2 | 1.488 | |
H3BO3 | 0.01 | g | 0.01 | 0.162 | |
Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
NaCl | 1.00 | g | 1 | 17.112 | |
Na2SeO3 x 5 H2O | 0.02 | g | 0.02 | 0.076 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve nitrilotriacetic acid at first and adjust pH to 6.5 with KOH. Then proceed to add the minerals. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 6.5 |
Equipment needed: | Autoclave |
Edit in Medium builder |
All strains for this medium: | 3 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Tryptone | 1.947 | 27.398 |
Glucose | 1.947 | 10.809 |
Yeast extract | 1.947 | - |
K2HPO4 | 1.461 | 8.386 |
NaCl | 0.885 | 15.146 |
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