Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
KH2PO4 | 0.75 | g | ||
|
K2HPO4 | 1.50 | g | ||
|
NaCl | 0.90 | g | ||
|
MgCl2 x 6 H2O | 0.20 | g | ||
|
NH4Cl | 0.90 | g | ||
|
Trace mineral solution | 9.00 | ml | ||
|
Trace vitamins | 10.00 | ml | ||
|
FeSO4 x 7 H2O
(10%) |
5.00 | �l | ||
|
Tryptone
(BD-Difco) |
2.00 | g | ||
|
Yeast extract | 2.00 | g | ||
|
Glucose | 2.00 | g | ||
|
Resazurin | 1.00 | mg | ||
|
Distilled water | 1000.00 | ml | ||
1 Mix components and adjust pH to 7.5. Bring to a boil, then cool down and distribute into culture vessels under a N2 gas stream. Seal with butyl rubber stoppers and autoclave. Prior to inoculation, add the following solution (autoclaved under a N2 atmosphere) into the medium (per liter): | |||||
|
Na2S x 9 H2O
(5%) |
8.00 | ml |
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Nitrilotriacetic acid | 12.50 | g | ||
|
FeCl2 x 4 H2O | 0.20 | g | ||
|
MnCl2 x 4 H2O | 0.10 | g | ||
|
CoCl2 x 6 H2O | 0.02 | g | ||
|
CaCl2 x 2 H2O | 0.10 | g | ||
|
ZnCl2 | 0.10 | g | ||
|
CuCl2 | 0.20 | g | ||
|
H3BO3 | 0.01 | g | ||
|
Na2MoO4 x 2 H2O | 0.01 | g | ||
|
NaCl | 1.00 | g | ||
|
Na2SeO3 x 5 H2O | 0.02 | g | ||
|
Distilled water | 1000.00 | ml | ||
1 Dissolve nitrilotriacetic acid at first and adjust pH to 6.5 with KOH. Then proceed to add the minerals. |
Compound | Amount | Unit | |
---|---|---|---|
|
Biotin | 2.0 | mg |
|
Folic acid | 2.0 | mg |
|
Pyridoxine hydrochloride | 10.0 | mg |
|
Thiamine HCl | 5.0 | mg |
|
Riboflavin | 5.0 | mg |
|
Nicotinic acid | 5.0 | mg |
|
Calcium pantothenate | 5.0 | mg |
|
Vitamin B12 | 0.1 | mg |
|
p-Aminobenzoic acid | 5.0 | mg |
|
Lipoic acid | 5.0 | mg |
|
Distilled water | 1000.0 | ml |