Name: ALICYCLOBACILLUS FERROOXYDANS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Main sol. J685 #

Solution A #

1000

Equipment needed: Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
MgSO₄ x 7 H₂O	0.50	g	0.5	2.029
(NH₄)₂SO₄	0.40	g	0.4	3.027
K₂HPO₄	0.20	g	0.2	1.148
K₂S₄O₆	0.15	g	0.15	-
KCl	0.10	g	0.1	1.341
Yeast extract	0.20	g	0.2	-
Distilled water	1000.00	ml	-	-
1 Adjust pH to 2.5 with H₂SO₄ and autoclave.

Equipment needed: Filter Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
FeSO₄ x 7 H₂O	20	g	-	-
1 Dissolve in final 100 ml of 0.2 N H₂SO₄ and filter-sterilize.
2 To complete the medium, add 70 ml Solution B to 1.0 L Solution A. For solid medium, prepare double-strength Solution A and autoclave. Separately autoclave an equal amount of 1.4% Gerlite (gellan gum) solution. After autoclaving, keep the two solutions at 70C and mix thoroughly. Then, add filter-sterilized Solution B (70 ml/L) and pour into petri dishes.