J685
685 .
ALICYCLOBACILLUS FERROOXYDANS MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
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Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MgSO4 x 7 H2O | 0.50 | g | 0.5 | 2.029 | |
| (NH4)2SO4 | 0.40 | g | 0.4 | 3.027 | |
| K2HPO4 | 0.20 | g | 0.2 | 1.148 | |
| K2S4O6 | 0.15 | g | 0.15 | - | |
| KCl | 0.10 | g | 0.1 | 1.341 | |
| Yeast extract | 0.20 | g | 0.2 | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 2.5 with H2SO4 and autoclave. | |||||
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| FeSO4 x 7 H2O | 20 | g | - | - | |
| 1 Dissolve in final 100 ml of 0.2 N H2SO4 and filter-sterilize. | |||||
| 2 To complete the medium, add 70 ml Solution B to 1.0 L Solution A. For solid medium, prepare double-strength Solution A and autoclave. Separately autoclave an equal amount of 1.4% Gerlite (gellan gum) solution. After autoclaving, keep the two solutions at 70C and mix thoroughly. Then, add filter-sterilized Solution B (70 ml/L) and pour into petri dishes. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 2.5 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
