Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 10.0 | g | 9.99 | 170.945 | |
Yeast extract | 2.0 | g | 1.998 | - | |
Trypticase peptone | 2.0 | g | 1.998 | - | |
NH4Cl | 1.0 | g | 0.999 | 18.676 | |
MgCl2 x 6 H2O | 1.0 | g | 0.999 | 4.914 | |
KCl | 0.5 | g | 0.5 | 6.7 | |
CaCl2 x 2 H2O | 0.4 | g | 0.4 | 2.718 | |
K2HPO4 | 0.4 | g | 0.4 | 2.294 | |
Resazurin | 0.5 | mg | 5.0e-4 | 0.002 | |
Trace minerals | 10.0 | ml | - | - | |
Selenite-tungstate solution | 1.0 | ml | - | - | |
NaHCO3 | 4.0 | g | 3.996 | 47.568 | |
Distilled water | 920.0 | ml | - | - | |
1 Mix components except NaHCO3, bring to a boil for several seconds and cool down under a stream of N2-CO2 (80:20). Add NaHCO3, mix thoroughly and distribute the medium into culture vessels under the same gas mixture. Seal with butyl rubber stoppers and autoclave. Finally, add per liter the following sterile solutions (autoclaved or *filter-sterilized): | |||||
Methanol solution (see below)
(*) |
50.0 | ml | - | - | |
Trace vitamins (see Medium No. 197)
(*) |
10.0 | ml | - | - | |
L-Cysteine HCl x H2O
(5%) |
5.0 | ml | - | - | |
Na2S x 9 H2O
(5%) |
5.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Methanol | 2.28 | ml | - | - |
Distilled water | 50.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
NaCl | 1.00 | g | 1 | 17.112 | |
FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
CoSO4 x 7 H2O | 0.10 | g | 0.1 | 0.645 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
H3BO3 | 0.01 | g | 0.01 | 0.162 | |
Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaOH | 0.4 | g | 0.4 | 10.001 |
Na2SeO3 x 5 H2O | 6.0 | mg | 0.006 | 0.023 |
Na2WO4 x 2 H2O | 8.0 | mg | 0.008 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 29.10.21 |
Source: | JCM |
Taxonomic range: | Archaea |
Medium type: | Complex medium |
Final pH: | n.d. |
Equipment needed: | Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 1 |