Name: SPIROCHAETA SMAEAGDINAE MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Main sol. J694 #

Solution A #

910

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NaCl	50.00	g	54.945	940.196
NH₄Cl	1.00	g	1.099	20.544
K₂HPO₄	0.33	g	0.363	2.082
KH₂PO₄	0.33	g	0.363	2.665
KCl	0.20	g	0.22	2.948
Yeast extract	5.00	g	5.495	-
Trace minerals	10.00	ml	-	-
L-Cysteine HCl x H₂O	0.50	g	0.549	-
Resazurin	1.00	mg	0.001	0.005
Distilled water	900.00	ml	-	-

104

Equipment needed: Autoclave Anaerobic workbench Filter

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
MgCl₂ x 6 H₂O	0.2	g	1.923	9.459
CaCl₂ x 2 H₂O	0.1	g	0.962	6.54
Distilled water	50.0	ml	-	-
1 Separately autoclave Solutions A and B under a N₂-CO₂ (4:1, v/v) gas mixture. After cooling, combine both solutions under the same gas mixture and add the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized):
NaHCO₃ (*, 8%)	50.0	ml	-	-
Na₂S x 9 H₂O (5%)	4.0	ml	-	-
2 Check the final pH of the medium to be 7.0.

1000

Solution occurs in 97 other media (first defined in Medium J151).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Nitrilotriacetic acid	1.50	g	1.5	7.848
MgSO₄ x 7 H₂O	3.00	g	3	12.172
MnSO₄ x n H₂O	0.50	g	0.5	-
NaCl	1.00	g	1	17.112
FeSO₄ x 7 H₂O	0.10	g	0.1	0.36
CoSO₄ x 7 H₂O	0.10	g	0.1	0.645
CaCl₂ x 2 H₂O	0.10	g	0.1	0.68
ZnSO₄ x 7 H₂O	0.10	g	0.1	0.348
CuSO₄ x 5 H₂O	0.01	g	0.01	0.04
AlK(SO₄)₂	0.01	g	0.01	0.039
H₃BO₃	0.01	g	0.01	0.162
Na₂MoO₄ x 2 H₂O	0.01	g	0.01	0.041
Distilled water	1000.00	ml	-	-
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Last modified:	22.02.22
Source:	JCM
Taxonomic range:	Bacteria
Medium type:	Complex medium
Final pH:	7.0
Equipment needed:	Anaerobic workbench, Autoclave, Filter

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