Name: NAUTILIA ABYSSI MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J695 #

Equipment needed: Anaerobic workbench Filter Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NaCl	25.00	g	23.81	407.418
NaNO₃	1.00	g	0.952	11.205
NH₄Cl	0.33	g	0.314	5.876
KCl	0.33	g	0.314	4.216
CaCl₂ x 2 H₂O	0.33	g	0.314	2.138
MgCl₂ x 6 H₂O	0.33	g	0.314	1.546
MES	1.95	g	1.857	9.512
Resazurin	1.00	mg	9.5e-4	0.004
Trace minerals	10.00	ml	-	-
Sulfur (powder)	12.00	g	11.429	356.363
Distilled water	1000.00	ml	-	-
1 Mix components except sulfur, adjust pH to 6.0 and autoclave under a N₂ atmosphere. Distribute the medium into culture vessels (e.g., 20 ml in 100 ml serum bottles) containing appropriate amount of sulfur under a H₂-CO₂ (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized) into the medium (per liter):
Trace vitamins (see Medium No. 197) (*)	10.00	ml	-	-
KH₂PO₄ (2%)	20.00	ml	-	-
Na₂S x 9 H₂O (5%)	10.00	ml	-	-
2 Pressurize the inoculated culture vessel to 200 kPa H₂-CO₂ (4:1, v/v).

Trace minerals #

1000

Solution occurs in 97 other media (first defined in Medium J151).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Nitrilotriacetic acid	1.50	g	1.5	7.848
MgSO₄ x 7 H₂O	3.00	g	3	12.172
MnSO₄ x n H₂O	0.50	g	0.5	-
NaCl	1.00	g	1	17.112
FeSO₄ x 7 H₂O	0.10	g	0.1	0.36
CoSO₄ x 7 H₂O	0.10	g	0.1	0.645
CaCl₂ x 2 H₂O	0.10	g	0.1	0.68
ZnSO₄ x 7 H₂O	0.10	g	0.1	0.348
CuSO₄ x 5 H₂O	0.01	g	0.01	0.04
AlK(SO₄)₂	0.01	g	0.01	0.039
H₃BO₃	0.01	g	0.01	0.162
Na₂MoO₄ x 2 H₂O	0.01	g	0.01	0.041
Distilled water	1000.00	ml	-	-
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Last modified:	22.02.22
Source:	JCM
Taxonomic range:	Bacteria
Medium type:	Defined medium
Final pH:	6.0
Equipment needed:	Anaerobic workbench, Autoclave, Filter

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