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JCM 695 . NAUTILIA ABYSSI MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Anaerobic workbench Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 25.00 g 23.81 407.418
NaNO3 1.00 g 0.952 11.205
NH4Cl 0.33 g 0.314 5.876
KCl 0.33 g 0.314 4.216
CaCl2 x 2 H2O 0.33 g 0.314 2.138
MgCl2 x 6 H2O 0.33 g 0.314 1.546
MES 1.95 g 1.857 9.512
Resazurin 1.00 mg 9.5e-4 0.004
Trace minerals 10.00 ml - -
Sulfur
(powder)
12.00 g 11.429 356.363
Distilled water 1000.00 ml - -
1 Mix components except sulfur, adjust pH to 6.0 and autoclave under a N2 atmosphere. Distribute the medium into culture vessels (e.g., 20 ml in 100 ml serum bottles) containing appropriate amount of sulfur under a H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized) into the medium (per liter):
Trace vitamins (see Medium No. 197)
(*)
10.00 ml - -
KH2PO4
(2%)
20.00 ml - -
Na2S x 9 H2O
(5%)
10.00 ml - -
2 Pressurize the inoculated culture vessel to 200 kPa H2-CO2 (4:1, v/v).
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.0
Equipment needed: Anaerobic workbench, Autoclave, Filter

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 23.819 407.581
Sulfur 11.429 356.363
MES 1.857 9.512
NaNO3 0.952 11.205
CaCl2 x 2 H2O 0.315 2.144
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain

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