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J696. THERMODESULFATATOR ATLANTICUS MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 20.00 g 19.608 335.507
2 3.00 g 2.941 14.467
3 3.00 g 2.941 20.706
4 0.50 g 0.49 6.575
5 0.30 g 0.294 5.498
6 0.10 g 0.098 0.72
7 1.73 g 1.696 -
8 0.11 g 0.108 0.734
9 Trace minerals 10.00 ml - -
10 Trace vitamins 10.00 ml - -
11 0.30 g 0.294 1.225
12 1000.00 ml 978.634 54322.4
Instructions:
  1. Mix components except Na2S x 9H2O and adjust pH to 6.7. Distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) under a gas stream of H2-CO2 (4:1, v/v), seal with butyl rubber stoppers and autoclave. Separately autoclave 3% Na2S x 9H2O solution under a N2 gas atmosphere. Prior to inoculation, reduce the medium by the addition of the Na2S x 9H2O solution. Pressurized the inoculated culture vessels to 200 kPa H2-CO2 (4:1, v/v).
Solution occurs in 88 other media (first defined in medium J151 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 7.848
2 3.00 g 3 12.171
3 0.50 g 0.5 -
4 1.00 g 1 17.111
5 0.10 g 0.1 0.36
6 0.10 g 0.1 -
7 0.10 g 0.1 0.68
8 0.10 g 0.1 -
9 0.01 g 0.01 -
10 0.01 g 0.01 0.039
11 0.01 g 0.01 0.162
12 0.01 g 0.01 0.041
13 1000.00 ml 998.207 55408.8
Instructions:
  1. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
NaCl 19.618 335.675
Na2SO4 2.941 20.706
MgCl2 x 6H2O 2.941 14.467
PIPES 1.696 -
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