696 .
THERMODESULFATATOR ATLANTICUS MEDIUM
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Equipment needed:
Autoclave
Final pH:
6.7
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
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NaCl | 20.00 | g | ||
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MgCl2 x 6 H2O | 3.00 | g | ||
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Na2SO4 | 3.00 | g | ||
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KCl | 0.50 | g | ||
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NH4Cl | 0.30 | g | ||
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KH2PO4 | 0.10 | g | ||
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PIPES | 1.73 | g | ||
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CaCl2 x 2 H2O | 0.11 | g | ||
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Trace minerals | 10.00 | ml | ||
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Trace vitamins | 10.00 | ml | ||
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Na2S x 9 H2O | 0.30 | g | ||
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Distilled water | 1000.00 | ml | ||
| 1 Mix components except Na2S x 9H2O and adjust pH to 6.7. Distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) under a gas stream of H2-CO2 (4:1, v/v), seal with butyl rubber stoppers and autoclave. Separately autoclave 3% Na2S x 9H2O solution under a N2 gas atmosphere. Prior to inoculation, reduce the medium by the addition of the Na2S x 9H2O solution. Pressurized the inoculated culture vessels to 200 kPa H2-CO2 (4:1, v/v). | |||||
Trace minerals #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nitrilotriacetic acid | 1.50 | g | ||
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MgSO4 x 7 H2O | 3.00 | g | ||
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MnSO4 x n H2O | 0.50 | g | ||
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NaCl | 1.00 | g | ||
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FeSO4 x 7 H2O | 0.10 | g | ||
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CoSO4 x 7 H2O | 0.10 | g | ||
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CaCl2 x 2 H2O | 0.10 | g | ||
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ZnSO4 x 7 H2O | 0.10 | g | ||
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CuSO4 x 5 H2O | 0.01 | g | ||
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AlK(SO4)2 | 0.01 | g | ||
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H3BO3 | 0.01 | g | ||
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Na2MoO4 x 2 H2O | 0.01 | g | ||
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Distilled water | 1000.00 | ml | ||
| 1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. | |||||
Trace vitamins #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
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Pyridoxine hydrochloride | 10.0 | mg |
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Thiamine HCl | 5.0 | mg |
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Riboflavin | 5.0 | mg |
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Nicotinic acid | 5.0 | mg |
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Calcium pantothenate | 5.0 | mg |
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Vitamin B12 | 0.1 | mg |
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p-Aminobenzoic acid | 5.0 | mg |
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Lipoic acid | 5.0 | mg |
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Distilled water | 1000.0 | ml |