J698
698 .
ACIDIMICROBIUM MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J698 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MgSO4 x 7 H2O | 0.5 | g | 0.499 | 2.025 | |
| (NH4)2SO4 | 0.4 | g | 0.399 | 3.021 | |
| K2HPO4 | 0.2 | g | 0.2 | 1.146 | |
| KCl | 0.1 | g | 0.1 | 1.339 | |
| FeSO4 x 7 H2O | 10.0 | mg | 0.01 | 0.036 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 2.0 with H2SO4. After autoclaving, add the following solution (autoclaved): | |||||
|
Yeast extract
(10%, BD-Difco) |
2.5 | ml | - | - | |
| 2 Comment: For autotrophic growth on ferrous sulfate, prepare the medium without yeast extract, add 13.9 g/L (final) FeSO4 x 7H2O and adjust pH to 1.7 with H2SO4. | |||||
Metadata
| Last modified: | 24.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 1.7 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| MgSO4 x 7 H2O | 0.499 | 2.025 |
| (NH4)2SO4 | 0.399 | 3.021 |
| K2HPO4 | 0.2 | 1.146 |
| KCl | 0.1 | 1.339 |
| FeSO4 x 7 H2O | 0.01 | 0.036 |
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