JCM 698 . ACIDIMICROBIUM MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 0.5 g 0.499 2.025
(NH4)2SO4 0.4 g 0.399 3.021
K2HPO4 0.2 g 0.2 1.146
KCl 0.1 g 0.1 1.339
FeSO4 x 7 H2O 10.0 mg 0.01 0.036
Distilled water 1000.0 ml - -
1 Adjust pH to 2.0 with H2SO4. After autoclaving, add the following solution (autoclaved):
Yeast extract
(10%, BD-Difco)
2.5 ml - -
2 Comment: For autotrophic growth on ferrous sulfate, prepare the medium without yeast extract, add 13.9 g/L (final) FeSO4 x 7H2O and adjust pH to 1.7 with H2SO4.

Metadata

Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 1.7
Equipment needed: Autoclave

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 0.499 2.025
(NH4)2SO4 0.399 3.021
K2HPO4 0.2 1.146
KCl 0.1 1.339
FeSO4 x 7 H2O 0.01 0.036
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
nutrition autotroph 1 strain
nutrition heterotroph 1 strain
temperature thermophilic 1 strain