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JCM 708 . DEFINED MEDIUM FOR SULFATE-REDUCING BACTERIA WITH BUTYRATE

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J708 #

ml
Equipment needed: Filter Autoclave Anaerobic workbench
1 Mix ingredients and adjust pH to 7.5. Distribute the medium into culture vessels under a N2-CO2 (95:5, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. Anaerobically and aseptically add per liter the following solutions (autoclaved or *filter-sterilized) to the medium:
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.5 g 0.499 3.667
NH4Cl 1.0 g 0.998 18.658
CaCl2 x 2 H2O 0.1 g 0.1 0.679
MgSO4 x 7 H2O 2.5 g 2.495 10.123
NaCl 1.0 g 0.998 17.077
FeCl2 solution 10.0 ml - -
Trace element solution 10.0 ml - -
Resazurin 1.0 mg 1.0e-3 0.004
Distilled water 870.0 ml - -
Sodium butyrate
(2.2%) 		100.0 ml - -
NaHCO3
(*, 8%) 		2.5 ml - -
L-Cysteine HCl x H2O
(5%) 		10.0 ml - -
2 Readjust pH of the medium to 7.5, if necessary.
3 For preparation of solid medium, add 15.0 g/L agar (BD-Difco).

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.5
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 2.495 10.123
NH4Cl 0.998 18.658
NaCl 0.998 17.077
KH2PO4 0.499 3.667
CaCl2 x 2 H2O 0.1 0.679
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Cultivation metadata from 2 strains BacDive

pH 6 - 8 1 strain
phyla 1 2 strains
oxygen anaerobe 1 strain
temperature mesophilic 2 strains