J716
716 .
USAB 53 MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J716 #
ml
Equipment needed: Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 1.00 | g | 0.999 | 18.676 | |
| KH2PO4 | 0.30 | g | 0.3 | 2.202 | |
| K2HPO4 | 0.30 | g | 0.3 | 1.721 | |
| NaCl | 25.00 | g | 24.975 | 427.362 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| KCl | 0.10 | g | 0.1 | 1.34 | |
| Na2SO4 | 2.84 | g | 2.837 | 19.974 | |
| Trace mineral solution | 1.00 | ml | - | - | |
| Yeast extract | 1.00 | g | 0.999 | - | |
| Sodium lactate | 2.24 | g | 2.238 | 19.969 | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix components and adjust pH to 6.8 - 7.0 with NaOH. Bring to a boil, cool down under a N2 gas stream and add 2 ml of 5% Na2S x 9H2O solution. Anaerobically distribute the medium into culture vessels, seal with butyl rubber stoppers and autoclave. | |||||
Solution occurs in 4
other media (first defined in Medium J684).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 12.50 | g | 12.5 | 65.397 | |
| FeCl2 x 4 H2O | 0.20 | g | 0.2 | 1.006 | |
| MnCl2 x 4 H2O | 0.10 | g | 0.1 | - | |
| CoCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnCl2 | 0.10 | g | 0.1 | 0.734 | |
| CuCl2 | 0.20 | g | 0.2 | 1.488 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| Na2SeO3 x 5 H2O | 0.02 | g | 0.02 | 0.076 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve nitrilotriacetic acid at first and adjust pH to 6.5 with KOH. Then proceed to add the minerals. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 6.9 |
| Equipment needed: | Anaerobic workbench, Autoclave |
| Edit in Medium builder | |