Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 0.75 | g | 0.737 | 5.414 | |
K2HPO4 | 1.50 | g | 1.473 | 8.46 | |
NaCl | 0.90 | g | 0.884 | 15.128 | |
MgCl2 x 6 H2O | 0.20 | g | 0.196 | 0.966 | |
NH4Cl | 0.90 | g | 0.884 | 16.528 | |
Trace mineral solution | 9.00 | ml | - | - | |
Trace vitamins | 5.00 | ml | - | - | |
FeSO4 x 7 H2O
(10%) |
5.00 | �l | - | - | |
Tryptone
(BD-Difco) |
2.00 | g | 1.965 | 27.64 | |
Yeast extract | 2.00 | g | 1.965 | - | |
Casamino acids
(BD-Difco) |
2.00 | g | 1.965 | - | |
Resazurin | 1.00 | mg | 9.8e-4 | 0.004 | |
Distilled water | 1000.00 | ml | - | - | |
1 Mix components thoroughly. Bring to a boil, cool down and distribute into culture vessels under a N2 gas stream. Seal with butyl rubber stoppers and autoclave. Prior to inoculation, add the following solution (autoclaved under a N2 atmosphere) into the medium (per liter): | |||||
Na2S x 9 H2O
(5%) |
4.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 12.50 | g | 12.5 | 65.397 | |
FeCl2 x 4 H2O | 0.20 | g | 0.2 | 1.006 | |
MnCl2 x 4 H2O | 0.10 | g | 0.1 | - | |
CoCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
ZnCl2 | 0.10 | g | 0.1 | 0.734 | |
CuCl2 | 0.20 | g | 0.2 | 1.488 | |
H3BO3 | 0.01 | g | 0.01 | 0.162 | |
Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
NaCl | 1.00 | g | 1 | 17.112 | |
Na2SeO3 x 5 H2O | 0.02 | g | 0.02 | 0.076 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve nitrilotriacetic acid at first and adjust pH to 6.5 with KOH. Then proceed to add the minerals. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 29.10.21 |
Source: | JCM |
Medium type: | Complex medium |
Final pH: | n.d. |
Equipment needed: | Autoclave |
Edit in Medium builder |