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JCM 721 . THIOALBUS DENITRIFICANS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J721 #

ml
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 20.0 g 19.666 336.511
MgCl2 x 6 H2O 0.4 g 0.393 1.935
CaCl2 x 2 H2O 0.1 g 0.098 0.669
NH4Cl 0.2 g 0.197 3.677
KH2PO4 1.0 g 0.983 7.225
KCl 0.5 g 0.492 6.595
Yeast extract 0.1 g 0.098 -
Distilled water 1000.0 ml - -
1 Mix components and adjust pH to 7.5. Dispense the medium into culture vessels, purge with N2 for 5 min, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions (filter-sterilized and stored anaerobically):
Vitamin solution 1.0 ml - -
Trace element solution 1.0 ml - -
NaHCO3
(8%) 		5.0 ml - -
NaNO3
(1 M) 		5.0 ml - -
Na2S2O3
(1 M) 		5.0 ml - -

Vitamin solution #

100
Solution occurs in 14 other media (first defined in Medium J403).
Compound Amount Unit Conc. [g/L] Conc. [mM]
p-Aminobenzoic acid 4 mg 0.04 0.292
Biotin 1 mg 0.01 0.041
Nicotinic acid 10 mg 0.1 0.812
DL-Calcium pantothenate 5 mg 0.05 0.105
Pyridoxine hydrochloride 10 mg 0.1 -
Sodium phosphate buffer
(10 mM, pH 7.1) 		100 ml - -

Trace element solution #

1000
Solution occurs in 38 other media (first defined in Medium J439).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		12.5 ml - -
FeSO4 x 7 H2O 2.1 g 2.1 7.554
H3BO3 30.0 mg 0.03 0.485
MnCl2 x 4 H2O 100.0 mg 0.1 -
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
ZnSO4 x 7 H2O 144.0 mg 0.144 0.501
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 987.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 19.666 336.511
KH2PO4 0.983 7.225
KCl 0.492 6.595
MgCl2 x 6 H2O 0.393 1.935
NH4Cl 0.197 3.677
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen microaerophile 1 strain
temperature mesophilic 1 strain