730 .
RYE-BRAN MRS
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Equipment needed:
Filter
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Lactobacilli MRS broth
(BD-Difco) |
55 | g |
|
|
Rye-bran extract | 900 | ml |
|
|
Distilled water | 100 | ml |
Rye-bran extract #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Rye-bran | 40.0 | g | ||
|
|
Malted wheat meal | 2.0 | g | ||
|
|
Trypsin
(Sigma-Aldrich) |
0.8 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Mix components and incubate at 50C for 24 hr. Clarify the resulting turbid liquid by centrifugation (500 x g, 15 min) followed by paper-filtration. | |||||