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J731. SPOROMUSA MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.50 g 0.579 10.831
2 0.50 g 0.579 2.351
3 0.25 g 0.29 1.971
4 2.25 g 2.607 44.611
5 Trace vitamins 10.00 ml - -
6 FeCl2 solution 1.00 ml - -
7 Trace element solution 1.00 ml - -
8 Se/W solution 1.00 ml - -
9 6.70 g 7.764 66.273
10 2.00 g 2.318 -
11
(BD-Difco)
2.00 g 2.318 -
12 1.00 mg 0.001 0.005
13 850.00 ml 983.17 54574.2
Instructions:
  1. Autoclave under a N2 gas atmosphere.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.348 g 3.48 19.98
2 0.227 g 2.27 16.681
3 100.000 ml 998.207 55408.8
Instructions:
  1. Autoclave under a N2 gas atmosphere.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 4 g 57.143 680.219
2 50 ml 713.005 39577.7
3
(3%)
10 ml - -
4
(3%)
10 ml - -
Instructions:
  1. Filter-sterilize and store under a N2-CO2 (4:1, v/v) atmosphere.
  2. To complete the medium, combine Solutions A, B and C, and replace the gas phase with a N2-CO2 (4:1, v/v) gas mixture. Finally, add the following solutions from anaerobic stocks: #3-4
  3. Adjust pH of the medium to 7.0, if necessary.
Solution occurs in 89 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 70 mg 0.07 0.514
2 100 mg 0.1 -
3 6 mg 0.006 0.097
4 190 mg 0.19 -
5 2 mg 0.002 -
6 24 mg 0.024 -
7 36 mg 0.036 0.149
8 1000 ml 998.207 55408.8
Solution occurs in 87 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(7.7 M, 25%)
10.0 ml 3.17 86.943
2 1.5 g 1.5 -
3 990.0 ml 988.225 54854.8
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 4 other media (first defined in medium J317 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 4 mg 0.004 0.023
2 4 mg 0.004 -
3 1000 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

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