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JCM 731 . SPOROMUSA MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Solution A #

863
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.50 g 0.579 10.832
MgSO4 x 7 H2O 0.50 g 0.579 2.351
CaCl2 x 2 H2O 0.25 g 0.29 1.97
NaCl 2.25 g 2.607 44.613
Trace vitamins 10.00 ml - -
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
Se/W solution 1.00 ml - -
Betaine 6.70 g 7.764 66.271
Yeast extract 2.00 g 2.318 -
Casitone
(BD-Difco) 		2.00 g 2.318 -
Resazurin 1.00 mg 0.001 0.005
Distilled water 850.00 ml - -
1 Autoclave under a N2 gas atmosphere.

Solution B #

100
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.348 g 3.48 19.98
KH2PO4 0.227 g 2.27 16.681
Distilled water 100.000 ml - -
1 Autoclave under a N2 gas atmosphere.

Solution C #

70
Equipment needed: Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 4 g 57.143 680.215
Distilled water 50 ml - -
1 To complete the medium, combine Solutions A, B and C, and replace the gas phase with a N2-CO2 (4:1, v/v) gas mixture. Finally, add the following solutions from anaerobic stocks:
L-Cysteine HCl x H2O
(3%) 		10 ml - -
Na2S x 9 H2O
(3%) 		10 ml - -
2 Filter-sterilize and store under a N2-CO2 (4:1, v/v) atmosphere.
3 Adjust pH of the medium to 7.0, if necessary.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Se/W solution #

1000
Solution occurs in 4 other media (first defined in Medium J317).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SeO3 4 mg 0.004 -
Na2WO4 x 2 H2O 4 mg 0.004 0.012
Distilled water 1000 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain