JCM 731 . SPOROMUSA MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.50 g 0.579 10.832
MgSO4 x 7 H2O 0.50 g 0.579 2.351
CaCl2 x 2 H2O 0.25 g 0.29 1.97
NaCl 2.25 g 2.607 44.613
Trace vitamins 10.00 ml - -
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
Se/W solution 1.00 ml - -
Betaine 6.70 g 7.764 66.271
Yeast extract 2.00 g 2.318 -
Casitone
(BD-Difco)
2.00 g 2.318 -
Resazurin 1.00 mg 0.001 0.005
Distilled water 850.00 ml - -
1 Autoclave under a N2 gas atmosphere.
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.348 g 3.48 19.98
KH2PO4 0.227 g 2.27 16.681
Distilled water 100.000 ml - -
1 Autoclave under a N2 gas atmosphere.
Equipment needed: Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 4 g 57.143 680.215
Distilled water 50 ml - -
1 To complete the medium, combine Solutions A, B and C, and replace the gas phase with a N2-CO2 (4:1, v/v) gas mixture. Finally, add the following solutions from anaerobic stocks:
L-Cysteine HCl x H2O
(3%)
10 ml - -
Na2S x 9 H2O
(3%)
10 ml - -
2 Filter-sterilize and store under a N2-CO2 (4:1, v/v) atmosphere.
3 Adjust pH of the medium to 7.0, if necessary.
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 -
Thiamine HCl 5.0 mg 0.005 -
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -
Solution occurs in 4 other media (first defined in Medium J317).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SeO3 4 mg 0.004 -
Na2WO4 x 2 H2O 4 mg 0.004 0.012
Distilled water 1000 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain