Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
1 Mix components under a N2-CO2 (80:20, v/v) gas mixture and autoclave under the same gas mixture in bottles sealed with butyl rubber stoppers. After cooling, aseptically and anaerobically add the following solutions from anaerobic stocks (autoclaved or *filter-sterilized) to the medium: | |||||
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NH4Cl | 0.85 | g | 0.847 | 15.843 | |
NaCl | 0.85 | g | 0.847 | 14.501 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.678 | |
MgCl2 x 6 H2O | 0.28 | g | 0.279 | 1.373 | |
Trace metal solution | 1.00 | ml | - | - | |
Selenite-tungstate solution | 1.00 | ml | - | - | |
Yeast extract | 0.20 | g | 0.199 | - | |
Resazurin | 0.50 | mg | 5.0e-4 | 0.002 | |
Distilled water | 830.00 | ml | - | - | |
Phosphate solution | 25.00 | ml | - | - | |
Trace vitamins (see Medium No. 197)
(*) |
1.00 | ml | - | - | |
NaHCO3
(*, 8%) |
40.00 | ml | - | - | |
L-Sodium lactate
(*, 0.1 M) |
100.00 | ml | - | - | |
Na2S x 9 H2O
(5%) |
5.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
KH2PO4 | 0.41 | g | 4.1 | 30.128 |
Na2HPO4 | 0.43 | g | 4.3 | 30.29 |
Distilled water | 100.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
HCl
(32%) |
10.000 | ml | 3.168 | 86.898 |
FeCl2 x 4 H2O | 2.000 | g | 1.98 | 9.96 |
CoCl2 x 6 H2O | 0.250 | g | 0.248 | 1.906 |
MnCl2 x 4 H2O | 0.100 | g | 0.099 | 0.5 |
ZnCl2 | 0.070 | g | 0.069 | 0.508 |
H3BO3 | 6.000 | mg | 0.006 | 0.096 |
Na2MoO4 x 2 H2O | 0.040 | g | 0.04 | 0.164 |
NiCl2 x 6 H2O | 0.070 | g | 0.069 | 0.535 |
CuCl2 x 2 H2O | 2.000 | mg | 0.002 | 0.012 |
AlCl3 | 0.025 | g | 0.025 | 0.186 |
Na2WO4 x 2 H2O | 6.000 | mg | 0.006 | 0.018 |
Distilled water | 1000.000 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaOH | 0.4 | g | 0.4 | 10.001 |
Na2SeO3 x 5 H2O | 6.0 | mg | 0.006 | 0.023 |
Na2WO4 x 2 H2O | 8.0 | mg | 0.008 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 29.10.21 |
Source: | JCM |
Medium type: | Complex medium |
Final pH: | n.d. |
Equipment needed: | Anaerobic workbench, Autoclave, Filter |
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