Loading ...

J741. ACETOBACTERIUM MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.00 g 0.962 17.976
2 0.33 g 0.317 2.332
3 0.45 g 0.433 2.484
4 0.10 g 0.096 0.39
5 Trace element solution 20.00 ml - -
6 Trace vitamins 20.00 ml - -
7 2.00 g 1.923 -
8 10.00 g 9.615 -
9 1.00 mg 9.6e-4 0.004
10 10.00 g 9.615 114.46
11 0.50 g 0.481 2.737
12 0.50 g 0.481 2.002
13 1000.00 ml 959.814 53277.7
Instructions:
  1. Dissolve ingredients except NaHCO3, fructose, cysteine and sodium sulfide, bring to a boil for a few minutes and cool to room temperature under a N2-CO2 (80:20, v/v) gas mixture. Add NaHCO3 (solid) and equilibrate the medium with the gas until pH reaches ca. 7.4. Then distribute and autoclave under the same gas atmosphere. Before use, adjust pH to 8.2 by adding sterile anaerobic Na2CO3 solution (ca. 0.25 ml of 5% Na2CO3 per 10 ml medium) and add fructose, cysteine and sodium sulfide from anaerobic sterile stock solutions.
Solution occurs in 1 other media (first defined in medium J741 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 7.848
2 3.00 g 3 12.171
3 0.50 g 0.5 -
4 1.00 g 1 17.111
5 0.10 g 0.1 0.36
6 0.18 g 0.18 -
7 0.10 g 0.1 0.68
8 0.18 g 0.18 -
9 10.00 mg 0.01 -
10 20.00 mg 0.02 -
11 10.00 mg 0.01 0.162
12 10.00 mg 0.01 0.041
13 25.00 mg 0.025 -
14 0.30 mg 3.0e-4 -
15 1000.00 ml 998.207 55408.8
Instructions:
  1. Dissolve nitrilotriacetic acid at first and adjust pH to 6.5 with KOH, then add the remaining minerals.
  2. Adjust final pH of the medium to 7.0 with KOH.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
NaHCO3 9.615 114.46
Fructose 9.615 -
Yeast extract 1.923 -
NH4Cl 0.962 17.976
Show more

Similar media

J1319. MODIFIED ACETOBACTER MEDIUM 90 %
J1106. MARINE METHANOGENIC MEDIUM 83 %
J945. MB MEDIUM FOR METHANOBACTERIUM 82 %
J755. MMJ-S304 MEDIUM 82 %
J702. METHANOBACTERIUM MEDIUM (II) 82 %

Latest comments

No comments for this medium.