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JCM 741 . ACETOBACTERIUM MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J741 #

ml
Equipment needed: Anaerobic workbench Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.00 g 0.962 17.976
KH2PO4 0.33 g 0.317 2.332
K2HPO4 0.45 g 0.433 2.484
MgSO4 x 7 H2O 0.10 g 0.096 0.39
Trace element solution 20.00 ml - -
Trace vitamins 20.00 ml - -
Yeast extract 2.00 g 1.923 -
Fructose 10.00 g 9.615 53.371
Resazurin 1.00 mg 9.6e-4 0.004
NaHCO3 10.00 g 9.615 114.459
L-Cysteine HCl x H2O 0.50 g 0.481 -
Na2S x 9 H2O 0.50 g 0.481 2.002
Distilled water 1000.00 ml - -
1 Dissolve ingredients except NaHCO3, fructose, cysteine and sodium sulfide, bring to a boil for a few minutes and cool to room temperature under a N2-CO2 (80:20, v/v) gas mixture. Add NaHCO3 (solid) and equilibrate the medium with the gas until pH reaches ca. 7.4. Then distribute and autoclave under the same gas atmosphere. Before use, adjust pH to 8.2 by adding sterile anaerobic Na2CO3 solution (ca. 0.25 ml of 5% Na2CO3 per 10 ml medium) and add fructose, cysteine and sodium sulfide from anaerobic sterile stock solutions.

Trace element solution #

1000
Solution occurs in 1 other media (first defined in Medium J741).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 10.00 mg 0.01 0.04
AlK(SO4)2 x 12 H2O 20.00 mg 0.02 -
H3BO3 10.00 mg 0.01 0.162
Na2MoO4 x 2 H2O 10.00 mg 0.01 0.041
NiCl2 x 6 H2O 25.00 mg 0.025 0.193
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid at first and adjust pH to 6.5 with KOH, then add the remaining minerals.
2 Adjust final pH of the medium to 7.0 with KOH.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Anaerobic workbench, Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 9.615 114.459
Fructose 9.615 53.371
Yeast extract 1.923 -
NH4Cl 0.962 17.976
Na2S x 9 H2O 0.481 2.002
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain