J743
743 .
CLOSTRIDIUM THERMOSULFUROGENES MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J743 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.3 | g | 0.296 | 2.172 | |
| Na2HPO4 x 12 H2O | 5.3 | g | 5.222 | 36.783 | |
| NH4Cl | 1.0 | g | 0.985 | 18.419 | |
| MgCl2 x 6 H2O | 0.2 | g | 0.197 | 0.969 | |
| Trace element solution | 10.0 | ml | - | - | |
| FeSO4 x 7 H2O | 1.5 | mg | 0.001 | 0.005 | |
| Trace vitamins | 5.0 | ml | - | - | |
| Resazurin | 1.0 | mg | 9.9e-4 | 0.004 | |
| Yeast extract | 1.0 | g | 0.985 | - | |
| Glucose | 5.0 | g | 4.926 | 27.343 | |
| Na2S x 9 H2O | 0.5 | g | 0.493 | 2.051 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 6.0 - 6.5. | |||||
| 2 Prepare sodium sulfide separately as 5% (w/v) solution, autoclave under a nitrogen atmosphere and neutralize with 1 N HCl before use. Sterilize glucose separately. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 12.80 | g | 12.8 | 66.967 | |
| FeCl2 x 4 H2O | 0.20 | g | 0.2 | 1.006 | |
| MnCl2 x 4 H2O | 0.10 | g | 0.1 | - | |
| CoCl2 x 6 H2O | 0.17 | g | 0.17 | 1.309 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnCl2 | 0.10 | g | 0.1 | 0.734 | |
| CuCl2 | 20.00 | mg | 0.02 | 0.149 | |
| H3BO3 | 10.00 | mg | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 10.00 | mg | 0.01 | 0.041 | |
| NiCl2 x 6 H2O | 26.00 | mg | 0.026 | 0.201 | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| Na2SeO3 x 5 H2O | 20.00 | mg | 0.02 | 0.076 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 First, dissolve nitrilotriacetic acid and adjust to pH 6.5 with KOH. | |||||
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 6.5 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |