J745
745 .
MJYP2 MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J745 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MJ(-N) synthetic seawater | 1000.00 | ml | - | - | |
| NaNO3 | 0.50 | g | 0.495 | 5.824 | |
| NH4Cl | 0.25 | g | 0.248 | 4.628 | |
|
Yeast extract
(BD-Difco) |
1.00 | g | 0.99 | - | |
|
Tryptone
(BD-Difco) |
1.00 | g | 0.99 | 13.929 | |
| Trace vitamins | 10.00 | ml | - | - | |
| 1 Mix components, adjust pH to 7.0 and autoclave. Replace the gas phase with N2-O2 (99:1, v/v) gas mixture, and pressurize to 200 kPa with the same gas mixture. | |||||
Solution occurs in 12
other media (first defined in Medium J268).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaCl | 30.00 | g | 29.703 | 508.264 |
| K2HPO4 | 0.14 | g | 0.139 | 0.796 |
| CaCl2 x 2 H2O | 0.14 | g | 0.139 | 0.943 |
| MgSO4 x 7 H2O | 3.40 | g | 3.366 | 13.658 |
| MgCl2 x 6 H2O | 4.18 | g | 4.139 | 20.357 |
| KCl | 0.33 | g | 0.327 | 4.383 |
| NiCl2 x 6 H2O | 0.50 | mg | 5.0e-4 | 0.004 |
| Na2SeO3 x 5 H2O | 0.50 | mg | 5.0e-4 | 0.002 |
| Fe(NH4)2(SO4)2 x 6 H2O | 0.01 | g | 0.01 | - |
| Modified Wolin's mineral solution | 10.00 | ml | - | - |
| Distilled water | 1000.00 | ml | - | - |
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |