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DSMZ

JCM 753 . DEHALOSPIRILLUM MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
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Medium for Sulfurospirillum multivorans DSM 12446

This strain uses the standard medium without modifications.

Solution A #

1024
Equipment needed: Anaerobic workbench Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SO4 0.700 g 0.684 4.813
K2HPO4 0.200 g 0.195 1.121
NH4Cl 0.250 g 0.244 4.564
NaCl 0.250 g 0.244 4.178
MgCl2 x 6 H2O 0.400 g 0.391 1.921
KCl 0.500 g 0.488 6.55
CaCl2 x 2 H2O 0.150 g 0.146 0.996
Resazurin 0.500 mg 4.9e-4 0.002
Yeast extract 2.000 g 1.953 -
FeCl2 solution 1.000 ml - -
Trace element solution 1.000 ml - -
Selenite-tungstate solution 1.000 ml - -
L-Cysteine HCl x H2O 0.050 g 0.049 -
FeSO4 x 7 H2O 0.025 g 0.024 0.088
Distilled water 900.000 ml - -
1 Mix components except L-cysteine x HCl x H2O and FeSO4 x 7H2O. Bring to a boil for a few seconds and cool down under a N2 atmosphere. While gassing with N2, add L-cysteine x HCl x H2O and FeSO4 x 7H2O to the solution, distribute into culture vessels (e.g., 18 ml in 50 ml serum bottles) under a N2 atmosphere, then, seal butyl rubber stoppers and autoclave. Aseptically and anaerobically add per liter (final) the following sterile solutions (autoclaved or *filter-sterilized) from anaerobic stocks:
Potassium phosphate buffer , pH 7.5
(0.1 M) 		10.000 ml - -
Trace vitamins (see Medium No. 197)
(*) 		10.000 ml - -
Vitamin B12 solution (see Medium No. 403)
(*) 		1.000 ml - -
NaHCO3
(*, 8%) 		42.000 ml - -
Sodium pyruvate
(*, 25%) 		18.000 ml - -
Sodium fumarate
(*, 16%) 		40.000 ml - -
2 Adjust pH of the medium to 7.3 - 7.7, if necessary.

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Selenite-tungstate solution #

1000
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1
Sulfurospirillum multivorans DSM 12446

No modifications necessary for this strain.

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain