Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 30.00 | g | 29.586 | 506.259 | |
MgCl2 x 6 H2O | 6.50 | g | 6.41 | 31.531 | |
CaCl2 x 2 H2O | 0.50 | g | 0.493 | 3.354 | |
KCl | 0.33 | g | 0.325 | 4.365 | |
NH4Cl | 0.25 | g | 0.247 | 4.609 | |
K2HPO4 | 0.14 | g | 0.138 | 0.793 | |
MnCl2 x 4 H2O | 4.00 | mg | 0.004 | - | |
Na2SeO3 x 5 H2O | 4.00 | mg | 0.004 | 0.015 | |
NiCl2 x 6 H2O | 2.00 | mg | 0.002 | 0.015 | |
CoCl2 x 6 H2O | 2.00 | mg | 0.002 | 0.015 | |
ZnCl2 | 0.50 | mg | 4.9e-4 | 0.004 | |
Na2MoO4 x 2 H2O | 0.40 | mg | 3.9e-4 | 0.002 | |
FeCl2 x 4 H2O | 0.25 | mg | 2.5e-4 | 0.001 | |
Na2WO2 x 2 H2O | 0.10 | mg | 9.9e-5 | - | |
H3BO3 | 0.10 | mg | 9.9e-5 | 0.002 | |
Sulfur | 10.00 | g | 9.862 | 307.513 | |
Resazurin | 0.50 | mg | 4.9e-4 | 0.002 | |
Distilled water | 1000.00 | ml | - | - | |
1 Mix components except sulfur and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions from anaerobic stocks (filter-sterilized): | |||||
Trace vitamins | 1.00 | ml | - | - | |
NaHCO3
(8%) |
12.50 | ml | - | - | |
2 Separately steam sulfur for 3 hr on each of 3 successive days. | |||||
3 To complete the medium, distribute the solution into sterile culture vessels containing appropriate amount of sulfur (e.g., 5 ml solution and 0.05 g sulfur in Hungate tubes) under a H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Then, add Na2S x 9H2O solution from an anaerobic stock (final 0.1 g/L). After inoculation, pressurize the inoculated culture vessels to 200 kPa H2-CO2 (4:1, v/v). |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 29.10.21 |
Source: | JCM |
Medium type: | Defined medium |
Final pH: | n.d. |
Equipment needed: | Anaerobic workbench, Autoclave, Filter, Hungate tubes |
Edit in Medium builder |