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JCM 755 . MMJ-S304 MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J755 #

ml
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Modified MJ synthetic seawater 1000.0 ml - -
Yeast extract 1.0 g 0.492 -
Trace vitamins 10.0 ml - -
Resazurin 1.0 mg 4.9e-4 0.002
Distilled water 1000.0 ml - -
1 Mix components and adjust pH to 6.5 with NaOH. Bring to a boil for a few seconds and cool down under a stream of a N2-CO2 (4:1, v/v) gas mixture. Distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) under the same gas mixture, seal with butyl rubber stoppers and autoclave. Add per liter the following solutions (autoclaved or *filter-sterilize) from anaerobic stocks:
NaHCO3
(*, 8%) 		12.5 ml - -
Na2S x 9 H2O
(5%) 		10.0 ml - -
2 Adjust pH of the medium to 6.0 - 6.5, if necessary. Pressurize the inoculated bottles to 200 kPa N2-CO2 (4:1, v/v).

Modified MJ synthetic seawater #

1000
Solution occurs in 4 other media (first defined in Medium J492).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 30.00 g 30 513.347
K2HPO4 0.14 g 0.14 0.804
CaCl2 x 2 H2O 0.50 g 0.5 3.401
NH4Cl 0.50 g 0.5 9.348
MgSO4 x 7 H2O 3.40 g 3.4 13.794
MgCl2 x 6 H2O 4.18 g 4.18 20.56
KCl 0.33 g 0.33 4.427
FeSO4 x 7 H2O 0.01 g 0.01 0.036
NiCl2 x 6 H2O 1.00 mg 1.0e-3 0.008
Na2SeO3 x 5 H2O 1.00 mg 1.0e-3 0.004
Trace element solution 10.00 ml - -
Distilled water 1000.00 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.2
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 14.623 250.213
MgCl2 x 6 H2O 2.037 10.018
MgSO4 x 7 H2O 1.671 6.781
Yeast extract 0.492 -
CaCl2 x 2 H2O 0.244 1.66
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain