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JCM 756 . GEOBACTER DALTONII MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J756 #

ml
Equipment needed: Filter Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 1.00 g 0.988 16.909
MgCl2 x 6 H2O 0.40 g 0.395 1.944
CaCl2 x 2 H2O 0.10 g 0.099 0.672
NH4Cl 0.25 g 0.247 4.618
KH2PO4 2.00 g 1.976 14.522
KCl 0.50 g 0.494 6.627
Trace element solution 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Resazurin 0.50 mg 4.9e-4 0.002
Distilled water 940.00 ml - -
1 Mix components and autoclave under a N2 atmosphere. After cooling, replace the gas phase with a N2-CO2 (4:1, v/v) gas mixture and add the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized):
NaHCO3
(*, 8%) 		30.00 ml - -
Vitamin solution (see Medium No. 403)
(*) 		1.00 ml - -
Thiamine solution (see Medium No. 403)
(*) 		1.00 ml - -
Vitamin B12 solution (see Medium No. 403)
(*) 		1.00 ml - -
Sodium acetate
(1 M) 		10.00 ml - -
Sodium fumarate
(1 M) 		20.00 ml - -
2 Distribute the medium into sterile culture vessels (e.g., 20 ml of the medium in 50 ml serum bottles) under a N2-CO2 (4:1, v/v) gas mixture and seal with sterile butyl rubber stoppers. Adjust pH to 7.0, if necessary. Prior to use, add per liter the following solution from an anaerobic stock (autoclaved):
L-Cysteine HCl x H2O
(5%) 		7.00 ml - -

Trace element solution #

1000
Solution occurs in 38 other media (first defined in Medium J439).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		12.5 ml - -
FeSO4 x 7 H2O 2.1 g 2.1 7.554
H3BO3 30.0 mg 0.03 0.485
MnCl2 x 4 H2O 100.0 mg 0.1 -
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
ZnSO4 x 7 H2O 144.0 mg 0.144 0.501
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 987.0 ml - -

Selenite-tungstate solution #

1000
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
KH2PO4 1.976 14.522
NaCl 0.988 16.909
KCl 0.494 6.627
MgCl2 x 6 H2O 0.395 1.944
NH4Cl 0.247 4.618
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain