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JCM 757 . DESULFOVIBRIO MEXICANUS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J757 #

ml
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.0 g 0.942 17.604
KH2PO4 0.3 g 0.282 2.076
K2HPO4 0.3 g 0.282 1.622
MgCl2 x 6 H2O 0.2 g 0.188 0.926
CaCl2 x 2 H2O 0.1 g 0.094 0.64
KCl 0.1 g 0.094 1.263
NaCl 0.6 g 0.565 9.668
Yeast extract 1.0 g 0.942 -
FeCl2 solution 1.0 ml - -
Trace element solution 1.0 ml - -
L-Cysteine HCl x H2O 0.5 g 0.471 -
Resazurin 0.5 mg 4.7e-4 0.002
Distilled water 900.0 ml - -
1 Mix components and adjust pH to 7.0. Distribute the medium into tubes or bottles under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers, and autoclave. After cooling, add per liter (final) the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized):
NaHCO3
(*, 8%) 		50.0 ml - -
Substrate solution 100.0 ml - -
Na2S x 9 H2O
(3%) 		10.0 ml - -
2 Readjust pH of the medium to 7.0, if necessary.

Substrate solution #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
L-Sodium lactate 12.5 g 125 1115.47
Na2S2O3 x 5 H2O 12.5 g 125 503.655
Distilled water 100.0 ml - -

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
L-Sodium lactate 11.77 105.035
Na2S2O3 x 5 H2O 11.77 47.425
NH4Cl 0.942 17.604
Yeast extract 0.942 -
NaCl 0.565 9.668
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain