Name: DETHIOSULFOVIBRIO PEPTIDOVORANS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J759 #

Equipment needed: Autoclave Anaerobic workbench Filter

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NH₄Cl	1.0	g	0.976	18.239
KH₂PO₄	0.3	g	0.293	2.151
K₂HPO₄	0.3	g	0.293	1.68
MgCl₂ x 6 H₂O	3.0	g	2.927	14.396
CaCl₂ x 2 H₂O	0.1	g	0.098	0.664
KCl	0.1	g	0.098	1.309
NaCl	30.0	g	29.268	500.826
Sodium acetate	0.5	g	0.488	5.947
Yeast extract	1.0	g	0.976	-
Trypticase peptone (BD-BBL)	5.0	g	4.878	-
Trace minerals	10.0	ml	-	-
Resazurin	0.5	mg	4.9e-4	0.002
Distilled water	900.0	ml	-	-
1 Mix components and bubble with N₂-CO₂ (4:1, v/v) gas mixture. Distribute the medium into tubes or bottles under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized; per 900 ml of medium):
NaHCO₃ (*, 8%)	60.0	ml	-	-
Na₂S₂O₃ x 5 H₂O (*, 12.5%)	40.0	ml	-	-
Na₂S x 9 H₂O (3%)	15.0	ml	-	-
2 Readjust pH of the medium to 7.3, if necessary.

Trace minerals #

1000

Solution occurs in 97 other media (first defined in Medium J151).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Nitrilotriacetic acid	1.50	g	1.5	7.848
MgSO₄ x 7 H₂O	3.00	g	3	12.172
MnSO₄ x n H₂O	0.50	g	0.5	-
NaCl	1.00	g	1	17.112
FeSO₄ x 7 H₂O	0.10	g	0.1	0.36
CoSO₄ x 7 H₂O	0.10	g	0.1	0.645
CaCl₂ x 2 H₂O	0.10	g	0.1	0.68
ZnSO₄ x 7 H₂O	0.10	g	0.1	0.348
CuSO₄ x 5 H₂O	0.01	g	0.01	0.04
AlK(SO₄)₂	0.01	g	0.01	0.039
H₃BO₃	0.01	g	0.01	0.162
Na₂MoO₄ x 2 H₂O	0.01	g	0.01	0.041
Distilled water	1000.00	ml	-	-
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Last modified:	22.02.22
Source:	JCM
Taxonomic range:	Bacteria
Medium type:	Complex medium
Final pH:	7.3
Equipment needed:	Anaerobic workbench, Autoclave, Filter

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