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JCM 760 . DESULFURISPIRILLUM ALKALIPHILUM MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J760 #

ml
Equipment needed: Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 22.0 g 21.033 198.442
NaHCO3 8.0 g 7.648 91.042
NaCl 6.0 g 5.736 98.154
K2HPO4 0.5 g 0.478 2.744
Distilled water 1000.0 ml - -
1 Mix components and autoclave under a N2 atmosphere. Then add the following solutions from sterile anaerobic stocks:
MgSO4
(0.1 M) 		10.0 ml - -
Yeast extract
(1%) 		2.0 ml - -
Sodium acetate
(1.0 M) 		20.0 ml - -
KNO3
(1.0 M) 		10.0 ml - -
Trace element solution SL-4 1.0 ml - -
2 Mix well until clarified. Finally, add the following solution to the medium:
Na2S x 9 H2O
(5%) 		2.5 ml - -
3 Comment: Incubate with a 5-10% inoculum. The strain also grows in the medium with sulfur as an electron acceptor. In this case, use the above described medium, replacing KNO3 with 0.25 g/L NH4Cl and 2.0 g/L sulfur (separately autoclaved at 110C for 40 min).

Trace element solution SL-4 #

1000
Solution occurs in 21 other media (first defined in Medium J340).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 0.5 g 0.5 1.711
FeSO4 x 7 H2O 0.2 g 0.2 0.719
Trace element solution SL-6 100.0 ml - -
Distilled water 900.0 ml - -

Trace element solution SL-6 #

1000
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Anaerobic workbench, Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2CO3 21.033 198.442
NaHCO3 7.648 91.042
NaCl 5.736 98.154
K2HPO4 0.478 2.744
EDTA 4.8e-4 0.002
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain