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J779. MODIFIED HALOANAEROBIUM MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 75.0 g 1071.43 18333
2 8.8 g 125.714 510.038
3 1.0 g 14.286 191.623
4 10.0 g 142.857 -
5
(BD-BBL)
10.0 g 142.857 -
6 Trace vitamins 10.0 ml - -
7 Trace minerals 10.0 ml - -
8
(10%)
25.0 ml - -
9 Thioglycolate-ascorbate solution
(*)
25.0 ml - -
Instructions:
  1. Add components to distilled water and bring volume to 1.0 L. Adjust pH 8.0 with NaOH. Autoclave under a N2 atmosphere. After cooling, add anaerobically the following solutions (autoclaved or *filter-sterilized): #8-9
  2. Readjust pH to 8.0, if necessary.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 20 -
2 0.5 g 20 -
3 25.0 ml 998.207 55408.8
Instructions:
  1. Prepare just before use. Filter-sterilize.
Solution occurs in 88 other media (first defined in medium J151 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 7.848
2 3.00 g 3 12.171
3 0.50 g 0.5 -
4 1.00 g 1 17.111
5 0.10 g 0.1 0.36
6 0.10 g 0.1 -
7 0.10 g 0.1 0.68
8 0.10 g 0.1 -
9 0.01 g 0.01 -
10 0.01 g 0.01 0.039
11 0.01 g 0.01 0.162
12 0.01 g 0.01 0.041
13 1000.00 ml 998.207 55408.8
Instructions:
  1. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 1071.57 18335.4
Distilled water 641.705 35619.9
Yeast extract 142.857 -
Trypticase peptone 142.857 -
MgSO4 x 7H2O 126.143 511.777
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