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JCM 781 . CLOSTRIDIUM ACETATE 1 MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Anaerobic workbench Hungate tubes Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.3 g 0.282 2.074
K2HPO4 0.3 g 0.282 1.62
NH4Cl 1.0 g 0.941 17.587
NaCl 1.0 g 0.941 16.097
KCl 0.1 g 0.094 1.262
CaCl2 x 2 H2O 0.1 g 0.094 0.64
MgCl2 x 6 H2O 0.5 g 0.47 2.314
Trace element solution 10.0 ml - -
Yeast extract 1.0 g 0.941 -
L-Cysteine HCl x H2O 0.5 g 0.47 -
Resazurin 1.0 mg 9.4e-4 0.004
Distilled water 1000.0 ml - -
1 Mix components thoroughly, distribute the medium into culture vessels (e.g., 5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from anaerobic stocks (autoclaved or *filter-sterilized):
NaHCO3
(*, 8%)
25.0 ml - -
Fructose
(1 M)
20.0 ml - -
Na2S x 9 H2O
(5%)
8.0 ml - -
Solution occurs in 38 other media (first defined in Medium J439).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
12.5 ml - -
FeSO4 x 7 H2O 2.1 g 2.1 7.554
H3BO3 30.0 mg 0.03 0.485
MnCl2 x 4 H2O 100.0 mg 0.1 -
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
ZnSO4 x 7 H2O 144.0 mg 0.144 0.501
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 987.0 ml - -

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Anaerobic workbench, Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NH4Cl 0.941 17.587
NaCl 0.941 16.097
Yeast extract 0.941 -
MgCl2 x 6 H2O 0.47 2.314
L-Cysteine HCl x H2O 0.47 -
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