J781
781 .
CLOSTRIDIUM ACETATE 1 MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J781 #
ml
Equipment needed: Autoclave
Anaerobic workbench
Hungate tubes
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.3 | g | 0.282 | 2.074 | |
| K2HPO4 | 0.3 | g | 0.282 | 1.62 | |
| NH4Cl | 1.0 | g | 0.941 | 17.587 | |
| NaCl | 1.0 | g | 0.941 | 16.097 | |
| KCl | 0.1 | g | 0.094 | 1.262 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.094 | 0.64 | |
| MgCl2 x 6 H2O | 0.5 | g | 0.47 | 2.314 | |
| Trace element solution | 10.0 | ml | - | - | |
| Yeast extract | 1.0 | g | 0.941 | - | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.47 | - | |
| Resazurin | 1.0 | mg | 9.4e-4 | 0.004 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Mix components thoroughly, distribute the medium into culture vessels (e.g., 5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from anaerobic stocks (autoclaved or *filter-sterilized): | |||||
|
NaHCO3
(*, 8%) |
25.0 | ml | - | - | |
|
Fructose
(1 M) |
20.0 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
8.0 | ml | - | - | |
Solution occurs in 38
other media (first defined in Medium J439).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
|
HCl
(25%) |
12.5 | ml | - | - |
| FeSO4 x 7 H2O | 2.1 | g | 2.1 | 7.554 |
| H3BO3 | 30.0 | mg | 0.03 | 0.485 |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | - |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 |
| ZnSO4 x 7 H2O | 144.0 | mg | 0.144 | 0.501 |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 |
| Distilled water | 987.0 | ml | - | - |
Metadata
| Last modified: | 29.10.21 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter, Hungate tubes |
| Edit in Medium builder | |