JCM 782 . CALDINITRATIRUPTOR MICROAEROPHILUS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave Anaerobic workbench Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.30 g 0.284 2.09
K2HPO4 0.30 g 0.284 1.633
NH4Cl 1.00 g 0.948 17.721
KNO3 1.70 g 1.611 15.938
NaCl 1.00 g 0.948 16.22
KCl 0.10 g 0.095 1.271
CaCl2 x 2 H2O 0.10 g 0.095 0.645
MgCl2 x 6 H2O 0.25 g 0.237 1.166
Trace minerals 10.00 ml - -
Yeast extract 1.00 g 0.948 -
Distilled water 1000.00 ml - -
1 Mix components thoroughly, adjust pH to 7.2 with KOH and distribute the medium into culture vessels (e.g., 5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from anaerobic stocks (autoclaved or *filter-sterilized):
NaHCO3
(*, 8%)
25.00 ml - -
Glucose
(1 M)
20.00 ml - -
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Metadata

Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Anaerobic workbench, Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
KNO3 1.611 15.938
NaCl 0.957 16.382
NH4Cl 0.948 17.721
Yeast extract 0.948 -
KH2PO4 0.284 2.09
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain