Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 0.30 | g | 0.284 | 2.09 | |
K2HPO4 | 0.30 | g | 0.284 | 1.633 | |
NH4Cl | 1.00 | g | 0.948 | 17.721 | |
KNO3 | 1.70 | g | 1.611 | 15.938 | |
NaCl | 1.00 | g | 0.948 | 16.22 | |
KCl | 0.10 | g | 0.095 | 1.271 | |
CaCl2 x 2 H2O | 0.10 | g | 0.095 | 0.645 | |
MgCl2 x 6 H2O | 0.25 | g | 0.237 | 1.166 | |
Trace minerals | 10.00 | ml | - | - | |
Yeast extract | 1.00 | g | 0.948 | - | |
Distilled water | 1000.00 | ml | - | - | |
1 Mix components thoroughly, adjust pH to 7.2 with KOH and distribute the medium into culture vessels (e.g., 5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from anaerobic stocks (autoclaved or *filter-sterilized): | |||||
NaHCO3
(*, 8%) |
25.00 | ml | - | - | |
Glucose
(1 M) |
20.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
NaCl | 1.00 | g | 1 | 17.112 | |
FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
CoSO4 x 7 H2O | 0.10 | g | 0.1 | 0.645 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
H3BO3 | 0.01 | g | 0.01 | 0.162 | |
Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.2 |
Equipment needed: | Anaerobic workbench, Autoclave, Filter, Hungate tubes |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
KNO3 | 1.611 | 15.938 |
NaCl | 0.957 | 16.382 |
NH4Cl | 0.948 | 17.721 |
Yeast extract | 0.948 | - |
KH2PO4 | 0.284 | 2.09 |
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