Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
(NH4)2SO4 | 0.450 | g | 0.446 | 3.375 | |
KH2PO4 | 0.050 | g | 0.05 | 0.364 | |
MgSO4 x 7 H2O | 0.500 | g | 0.496 | 2.011 | |
KCl | 0.050 | g | 0.05 | 0.665 | |
Yeast extract | 0.500 | g | 0.496 | - | |
Ca(NO3)2 x 4 H2O | 0.014 | g | 0.014 | 0.059 | |
Na2SO4 | 3.000 | g | 2.973 | 20.932 | |
Trace element solution | 1.000 | ml | - | - | |
L-Cysteine HCl x H2O | 0.500 | g | 0.496 | - | |
Resazurin | 1.000 | mg | 9.9e-4 | 0.004 | |
Distilled water | 900.000 | ml | - | - | |
1 Mix components thoroughly, distribute the medium into culture vessels (e.g., 4.5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add the following solutions (per 900 ml medium) from anaerobic stocks (autoclaved or *filter-sterilized): | |||||
Na2HPO4
(5%) |
20.000 | ml | - | - | |
KH2PO4
(25%) |
40.000 | ml | - | - | |
Fructose
(1 M) |
20.000 | ml | - | - | |
Trace vitamins (see Medium No. 197)
(*) |
20.000 | ml | - | - | |
Na2S x 9 H2O
(5%) |
8.000 | ml | - | - | |
2 Check final pH of the medium to be around 5.8. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
HCl
(25%) |
12.5 | ml | - | - |
FeSO4 x 7 H2O | 2.1 | g | 2.1 | 7.554 |
H3BO3 | 30.0 | mg | 0.03 | 0.485 |
MnCl2 x 4 H2O | 100.0 | mg | 0.1 | - |
CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 |
NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 |
CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 |
ZnSO4 x 7 H2O | 144.0 | mg | 0.144 | 0.501 |
Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 |
Distilled water | 987.0 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 5.8 |
Equipment needed: | Anaerobic workbench, Autoclave, Filter, Hungate tubes |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Na2SO4 | 2.973 | 20.932 |
MgSO4 x 7 H2O | 0.496 | 2.011 |
Yeast extract | 0.496 | - |
L-Cysteine HCl x H2O | 0.496 | - |
(NH4)2SO4 | 0.446 | 3.375 |
Show more |