JCM 79 . METHANOL-UTILIZING BACTERIA MEDIUM B

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Solution occurs in 1 other media (first defined in Medium J79).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Methanol 10.0 ml - -
(NH4)2SO4 3.0 g 2.97 22.478
KH2PO4 1.4 g 1.386 10.186
Na2HPO4 3.0 g 2.97 20.924
MgSO4 x 7 H2O 0.2 g 0.198 0.803
Ferric citrate 30.0 mg 0.03 0.121
CaCl2 x 2 H2O 30.0 mg 0.03 0.202
MnCl2 x 4 H2O 5.0 mg 0.005 -
ZnSO4 x 7 H2O 5.0 mg 0.005 0.017
CuSO4 x 5 H2O 0.5 mg 5.0e-4 0.002
Thiamine HCl
(see below)
0.4 mg 4.0e-4 -
Distilled water 1000.0 ml - -
1 Thiamine x HCl can be replaced by 0.2 g of yeast extract.
2 Adjust pH to 7.1.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.1
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Associated strains

All strains for this medium: 63

Molecular composition

Compound Conc. [g/L] Conc. [mM]
(NH4)2SO4 2.97 22.478
Na2HPO4 2.97 20.924
KH2PO4 1.386 10.186
MgSO4 x 7 H2O 0.198 0.803
CaCl2 x 2 H2O 0.03 0.202
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Cultivation metadata from 63 strains BacDive

phyla 1 63 strains
oxygen aerobe 4 strains
oxygen obligate aerobe 3 strains
temperature mesophilic 63 strains
temperature psychrophilic 1 strain