Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 0.136 | g | 0.131 | 0.961 | |
NH4Cl | 0.540 | g | 0.519 | 9.707 | |
MgCl2 x 6 H2O | 0.200 | g | 0.192 | 0.946 | |
CaCl2 x 2 H2O | 0.147 | g | 0.141 | 0.961 | |
NaHCO3 | 2.500 | g | 2.404 | 28.615 | |
Yeast extract | 0.200 | g | 0.192 | - | |
Sodium acetate | 0.800 | g | 0.769 | 9.377 | |
Trace vitamins | 10.000 | ml | - | - | |
Trace minerals | 10.000 | ml | - | - | |
Resazurin | 1.000 | mg | 9.6e-4 | 0.004 | |
Distilled water | 1000.000 | ml | - | - | |
1 Mix components, bring to a boil for several seconds and cool down under a stream of H2-CO2 (80:20, v/v). Distribute the medium into culture vessels (e.g., 5 ml in the Hungate tube) under the same gas mixture, seal with butyl rubber stoppers and autoclave. Add per liter the following sterile solutions from anaerobic stocks (autoclaved under a N2 atmosphere): | |||||
L-Cysteine HCl x H2O
(5%) |
10.000 | ml | - | - | |
Na2S x 9 H2O
(5%) |
10.000 | ml | - | - | |
2 Pressurize the inoculated vessels to 150 kPa H2-CO2 (80:20, v/v). |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
NaCl | 1.00 | g | 1 | 17.112 | |
FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
CoSO4 x 7 H2O | 0.10 | g | 0.1 | 0.645 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
H3BO3 | 0.01 | g | 0.01 | 0.162 | |
Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. |
Last modified: | 29.10.21 |
Source: | JCM |
Taxonomic range: | Archaea |
Medium type: | Complex medium |
Final pH: | n.d. |
Equipment needed: | Anaerobic workbench, Autoclave, Hungate tubes |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaHCO3 | 2.404 | 28.615 |
Sodium acetate | 0.769 | 9.377 |
NH4Cl | 0.519 | 9.707 |
MgCl2 x 6 H2O | 0.192 | 0.946 |
Yeast extract | 0.192 | - |
Show more |