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J794. METHANOBACTERIUM MEDIUM (III)

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.136 g 0.131 0.961
2 0.540 g 0.519 9.707
3 0.200 g 0.192 0.946
4 0.147 g 0.141 0.961
5 2.500 g 2.404 28.615
6 0.200 g 0.192 -
7 0.800 g 0.769 9.377
8 Trace vitamins 10.000 ml - -
9 Trace minerals 10.000 ml - -
10 1.000 mg 9.6e-4 0.004
11 1000.000 ml 959.814 53277.7
12
(5%)
10.000 ml - -
13
(5%)
10.000 ml - -
Instructions:
  1. Mix components, bring to a boil for several seconds and cool down under a stream of H2-CO2 (80:20, v/v). Distribute the medium into culture vessels (e.g., 5 ml in the Hungate tube) under the same gas mixture, seal with butyl rubber stoppers and autoclave. Add per liter the following sterile solutions from anaerobic stocks (autoclaved under a N2 atmosphere): #12-13
  2. Pressurize the inoculated vessels to 150 kPa H2-CO2 (80:20, v/v).
Solution occurs in 88 other media (first defined in medium J151 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 7.848
2 3.00 g 3 12.171
3 0.50 g 0.5 -
4 1.00 g 1 17.111
5 0.10 g 0.1 0.36
6 0.10 g 0.1 -
7 0.10 g 0.1 0.68
8 0.10 g 0.1 -
9 0.01 g 0.01 -
10 0.01 g 0.01 0.039
11 0.01 g 0.01 0.162
12 0.01 g 0.01 0.041
13 1000.00 ml 998.207 55408.8
Instructions:
  1. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 979.01 54343.3
NaHCO3 2.404 28.615
Sodium acetate 0.769 9.377
NH4Cl 0.519 9.707
MgCl2 x 6H2O 0.192 0.946
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