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J814. TEPIDIMICROBIUM XYLANILYTICUM MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.49 g 0.544 4.001
2 1.19 g 1.322 9.314
3
(BD-Difco)
2.00 g 2.222 -
4 2.00 g 2.222 -
5 2.00 g 2.222 -
6 5.00 g 5.556 30.838
7 0.50 g 0.556 3.163
8 25.00 g 27.778 475.301
9 1.00 mg 0.001 0.005
10 900.00 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.30 g 2.632 49.197
2 0.10 g 0.877 4.315
3 0.01 g 0.088 0.597
4 50.00 ml 437.81 24302.1
5 Trace element solution 1.00 ml - -
6 FeCl2 solution 1.00 ml - -
7 Trace vitamins 10.00 ml - -
8
(8%)
50.00 ml 75.754 901.767
9
(5%)
2.00 ml - -
Instructions:
  1. Autoclave solutions A and B under a N2 atmosphere. After cooling, combine Solutions A and B together with the following solutions (filter-sterilized) under a N2-CO2 (4:1, v/v) gas mixture: #5-8
  2. Distribute the medium into culture vessels under the same gas mixture and seal with butyl rubber stoppers. Finally, add per liter the following solution (autoclaved and stored under a N2 atmosphere): #9
Solution occurs in 89 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 70 mg 0.07 0.514
2 100 mg 0.1 -
3 6 mg 0.006 0.097
4 190 mg 0.19 -
5 2 mg 0.002 -
6 24 mg 0.024 -
7 36 mg 0.036 0.149
8 1000 ml 998.207 55408.8
Solution occurs in 87 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(7.7 M, 25%)
10.0 ml 3.17 86.943
2 1.5 g 1.5 -
3 990.0 ml 988.225 54854.8
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 2

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