MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ

JCM 814 . TEPIDIMICROBIUM XYLANILYTICUM MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Solution A #

900
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.49 g 0.544 4.001
Na2HPO4 1.19 g 1.322 9.314
Tryptone
(BD-Difco) 		2.00 g 2.222 31.264
Yeast extract 2.00 g 2.222 -
Peptone 2.00 g 2.222 -
Glucose 5.00 g 5.556 30.837
L-Cysteine HCl x H2O 0.50 g 0.556 -
NaCl 25.00 g 27.778 475.321
Resazurin 1.00 mg 0.001 0.005
Distilled water 900.00 ml - -

Solution B #

114
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.30 g 2.632 49.198
MgCl2 x 6 H2O 0.10 g 0.877 4.315
CaCl2 x 2 H2O 0.01 g 0.088 0.597
Distilled water 50.00 ml - -
1 Autoclave solutions A and B under a N2 atmosphere. After cooling, combine Solutions A and B together with the following solutions (filter-sterilized) under a N2-CO2 (4:1, v/v) gas mixture:
Trace element solution 1.00 ml - -
FeCl2 solution 1.00 ml - -
Trace vitamins 10.00 ml - -
NaHCO3
(8%) 		50.00 ml - -
2 Distribute the medium into culture vessels under the same gas mixture and seal with butyl rubber stoppers. Finally, add per liter the following solution (autoclaved and stored under a N2 atmosphere):
Na2S x 9 H2O
(5%) 		2.00 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave, Filter
Edit in Medium builder

Associated strains

All strains for this medium: 2

Cultivation metadata from 2 strains BacDive

pH 4 - 6 2 strains
pH 8 - 10 2 strains
pH 6 - 8 1 strain
phyla 1 2 strains
oxygen anaerobe 2 strains
Show more