Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NH4Cl | 0.10 | g | 0.1 | 1.866 | |
MgSO4 x 7 H2O | 0.10 | g | 0.1 | 0.405 | |
CaCl2 x 2 H2O | 0.02 | g | 0.02 | 0.136 | |
Trace element solution | 0.10 | ml | - | - | |
Iron stock solution 4.5 g/L | 0.10 | ml | - | - | |
Distilled water | 1000.00 | ml | - | - | |
1 Mix components and autoclave. After cooling, add the following solution (autoclaved) to the medium: | |||||
Phosphate buffer stock solution | 2.00 | ml | - | - | |
2 To complete medium, adjust pH to 3.0 with sterile 1 N HCl solution. Then, distribute 20 ml-aliquots of the medium into sterile 120 ml-serum bottles and seal with butyl rubber stoppers. Add 100 ml methane gas (filter-sterilized) into the head space of the culture bottle through a syringe. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
ZnSO4 x 7 H2O | 0.400 | g | 0.4 | 1.391 |
Na2-EDTA | 0.250 | g | 0.25 | - |
MnCl2 x 4 H2O | 0.020 | g | 0.02 | - |
H3BO3 | 0.015 | g | 0.015 | 0.243 |
Na2MoO4 x 2 H2O | 0.040 | g | 0.04 | 0.165 |
NiCl2 x 6 H2O | 0.010 | g | 0.01 | 0.077 |
CuSO4 x 5 H2O | 0.200 | g | 0.2 | 0.801 |
CoCl2 x 6 H2O | 0.050 | g | 0.05 | 0.385 |
Distilled water | 1000.000 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Fe(III)-EDTA | 4.5 | g | 4.5 | - |
Distilled water | 1000.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
KH2PO4 | 37.425 | g | 37.425 | 275.01 |
Na2HPO4 x 2 H2O | 48.950 | g | 48.95 | 344.818 |
Distilled water | 1000.000 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Medium type: | Defined medium |
Final pH: | 3.0 |
Equipment needed: | Autoclave, Filter |
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