J819
819 .
BACILLUS MACYAE MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J819 #
ml
Equipment needed: Filter
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 1.20 | g | 1.186 | 20.29 | |
| KCl | 0.30 | g | 0.296 | 3.976 | |
| NH4Cl | 0.30 | g | 0.296 | 5.542 | |
| K2HPO4 | 0.20 | g | 0.198 | 1.135 | |
| Na2SO4 | 0.30 | g | 0.296 | 2.087 | |
| MgCl2 x 6 H2O | 0.40 | g | 0.395 | 1.944 | |
| CaCl2 x 2 H2O | 0.15 | g | 0.148 | 1.008 | |
| NaHCO3 | 0.60 | g | 0.593 | 7.058 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Resazurin | 0.50 | mg | 4.9e-4 | 0.002 | |
| Trace vitamins | 10.00 | ml | - | - | |
| Vitamin solution | 1.00 | ml | - | - | |
| Sodium lactate | 1.10 | g | 1.087 | 9.7 | |
| Yeast extract | 0.80 | g | 0.791 | - | |
| KNO3 | 0.50 | g | 0.494 | 4.887 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Prepare the medium anaerobically under 100% N2. Add vitamins (filter-sterilized), sodium lactate and nitrate from sterile, anaerobic stock solutions. Adjust final pH to 7.4 - 7.8. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Vitamin B12 | 100 | mg | 0.1 | 0.074 |
| p-Aminobenzoic acid | 80 | mg | 0.08 | 0.583 |
| Biotin | 20 | mg | 0.02 | 0.082 |
| Nicotinic acid | 200 | mg | 0.2 | 1.625 |
| Calcium pantothenate | 100 | mg | 0.1 | 0.21 |
| Pyridoxine hydrochloride | 300 | mg | 0.3 | - |
| Thiamine-HCl x 2 H2O | 200 | mg | 0.2 | - |
| Distilled water | 1000 | ml | - | - |
Solution occurs in 20
other media (first defined in Medium J433).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(25%, 7.7 M) |
10.0 | ml | 2.5 | 68.568 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 990.0 | ml | - | - | |
| 1 Dissolve FeCl2 x 4H2O first in HCl. Then dilute in distilled water and add the remaining salts. | |||||
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 7.6 |
| Equipment needed: | Anaerobic workbench, Filter |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .