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JCM 820 . METHANOTROPH 1A MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J820 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaNO3 1.00 g 0.999 11.754
MgSO4 x 7 H2O 0.10 g 0.1 0.405
Na2HPO4 x 12 H2O 0.40 g 0.4 -
KH2PO4 0.22 g 0.22 1.615
CaCl2 x 2 H2O 0.03 g 0.03 0.204
FeSO4 x 7 H2O 2.00 mg 0.002 0.007
Trace metal solution
(see below) 		1.00 ml - -
Distilled water 1000.00 ml - -
1 Adjust pH to 6.5.
2 Distribute the medium into culture vessels (leave >80% volume gas phase), seal with butyl rubber stoppers and autoclave. After cooling, add filter-sterilized methane gas to make a final concentration about 20% (v/v) methane in the gas phase.

Trace mineral solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.44 g 0.44 1.53
CuSO4 x 5 H2O 0.20 g 0.2 0.801
MnSO4 x n H2O 0.17 g 0.17 -
Na2MoO4 x 2 H2O 0.06 g 0.06 0.248
H3BO3 0.10 g 0.1 1.617
CoCl2 x 6 H2O 0.08 g 0.08 0.616
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.5
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaNO3 0.999 11.754
Na2HPO4 x 12 H2O 0.4 -
KH2PO4 0.22 1.615
MgSO4 x 7 H2O 0.1 0.405
CaCl2 x 2 H2O 0.03 0.204
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Cultivation metadata from 3 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 3 strains
oxygen aerobe 2 strains
temperature mesophilic 3 strains