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JCM 822 . FERMENTATIVE CELLULOLYTIC ANAEROBES MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J822 #

ml
Equipment needed: Filter Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
KCl 0.33 g 0.33 4.422
NH4Cl 0.25 g 0.25 4.669
MgSO4 x 7 H2O 0.60 g 0.599 2.432
NaCl 0.40 g 0.4 6.838
CaCl2 x 2 H2O 0.10 g 0.1 0.68
Trace minerals 10.00 ml - -
Yeast extract 0.20 g 0.2 -
Resazurin 0.25 mg 2.5e-4 0.001
1 M Phosphate buffer
(1.0 M) 		1.00 ml - -
MOPS 2.09 g 2.088 9.977
Distilled water 910.00 ml - -
1 Mix components and bring to a boil for 5-10 sec, then, cool down under a N2-CO2 (80:20, v/v) gas stream. Dispense the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized):
Cellobiose
(*, 10%) 		40.00 ml - -
Trace vitamins (see Medium No. 197)
(*) 		10.00 ml - -
NaHCO3
(*, 5%) 		10.00 ml - -
Na2S x 9 H2O
(5%) 		10.00 ml - -
L-Cysteine HCl x H2O
(5%) 		10.00 ml - -

1 M Phosphate buffer #

Solution occurs in 2 other media (first defined in Medium J556).
1 Mix 28 ml of 1 M KH2PO4 and 78 ml of 1 M Na2HPO4 to prepare 100 ml of 1 M phosphate buffer.

Trace minerals #

1000
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MOPS 2.088 9.977
MgSO4 x 7 H2O 0.629 2.553
NaCl 0.41 7.009
KCl 0.33 4.422
NH4Cl 0.25 4.669
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
nutrition chemoorganoheterotroph 1 strain
oxygen anaerobe 1 strain
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