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JCM 823 . TEPIDIBACTER MESOPHILUS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J823 #

ml
Equipment needed: Filter Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 5.00 g 4.869 83.309
MgCl2 x 6 H2O 4.00 g 3.895 19.158
KCl 0.34 g 0.331 4.441
NH4Cl 0.25 g 0.243 4.551
CaCl2 x 2 H2O 0.11 g 0.107 0.729
K2HPO4 0.18 g 0.175 1.006
Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg 0.002 -
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
Yeast extract 0.20 g 0.195 -
Casitone
(BD-Difco) 		2.00 g 1.947 -
Trace vitamins 10.00 ml - -
Resazurin 1.00 mg 9.7e-4 0.004
Distilled water 1000.00 ml - -
1 Mix components and bring to a boil for 5-10 sec and cool down under a N2-CO2 (80:20, v/v) gas stream. Dispense the medium into culture vessels (e.g., 20 ml in 50 ml serum bottles) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from sterile anaerobic stocks:
Na2S x 9 H2O
(5%) 		10.00 ml - -
NaHCO3
(8%) 		5.00 ml - -
2 To prepare the stock solutions, autoclave 5% Na2S x 9H2O solution under a N2 atmosphere, filter-sterilize and store 8% NaHCO3 solution under a N2-CO2 (80:20, v/v) gas mixture.

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 4.869 83.309
MgCl2 x 6 H2O 3.895 19.158
Casitone 1.947 -
KCl 0.331 4.441
NH4Cl 0.243 4.551
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
oxygen facultative anaerobe 1 strain
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