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J823. TEPIDIBACTER MESOPHILUS MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.00 g 4.869 83.305
2 4.00 g 3.895 19.158
3 0.34 g 0.331 4.441
4 0.25 g 0.243 4.551
5 0.11 g 0.107 0.729
6 0.18 g 0.175 1.006
7 2.00 mg 0.002 -
8 FeCl2 solution 1.00 ml - -
9 Trace element solution 1.00 ml - -
10 0.20 g 0.195 -
11
(BD-Difco)
2.00 g 1.947 -
12 Trace vitamins 10.00 ml - -
13 1.00 mg 9.7e-4 0.004
14 1000.00 ml 971.964 53952.1
15
(5%)
10.00 ml - -
16
(8%)
5.00 ml 0.841 10.01
Instructions:
  1. Mix components and bring to a boil for 5-10 sec and cool down under a N2-CO2 (80:20, v/v) gas stream. Dispense the medium into culture vessels (e.g., 20 ml in 50 ml serum bottles) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from sterile anaerobic stocks: #15-16
  2. To prepare the stock solutions, autoclave 5% Na2S x 9H2O solution under a N2 atmosphere, filter-sterilize and store 8% NaHCO3 solution under a N2-CO2 (80:20, v/v) gas mixture.
Solution occurs in 89 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 70 mg 0.07 0.514
2 100 mg 0.1 -
3 6 mg 0.006 0.097
4 190 mg 0.19 -
5 2 mg 0.002 -
6 24 mg 0.024 -
7 36 mg 0.036 0.149
8 1000 ml 998.207 55408.8
Solution occurs in 87 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(7.7 M, 25%)
10.0 ml 3.17 86.943
2 1.5 g 1.5 -
3 990.0 ml 988.225 54854.8
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 983.618 54599
NaCl 4.869 83.305
MgCl2 x 6H2O 3.895 19.158
Casitone 1.947 -
NaHCO3 0.841 10.01
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