Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 5.00 | g | 4.869 | 83.309 | |
MgCl2 x 6 H2O | 4.00 | g | 3.895 | 19.158 | |
KCl | 0.34 | g | 0.331 | 4.441 | |
NH4Cl | 0.25 | g | 0.243 | 4.551 | |
CaCl2 x 2 H2O | 0.11 | g | 0.107 | 0.729 | |
K2HPO4 | 0.18 | g | 0.175 | 1.006 | |
Fe(NH4)2(SO4)2 x 6 H2O | 2.00 | mg | 0.002 | - | |
FeCl2 solution | 1.00 | ml | - | - | |
Trace element solution | 1.00 | ml | - | - | |
Yeast extract | 0.20 | g | 0.195 | - | |
Casitone
(BD-Difco) |
2.00 | g | 1.947 | - | |
Trace vitamins | 10.00 | ml | - | - | |
Resazurin | 1.00 | mg | 9.7e-4 | 0.004 | |
Distilled water | 1000.00 | ml | - | - | |
1 Mix components and bring to a boil for 5-10 sec and cool down under a N2-CO2 (80:20, v/v) gas stream. Dispense the medium into culture vessels (e.g., 20 ml in 50 ml serum bottles) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from sterile anaerobic stocks: | |||||
Na2S x 9 H2O
(5%) |
10.00 | ml | - | - | |
NaHCO3
(8%) |
5.00 | ml | - | - | |
2 To prepare the stock solutions, autoclave 5% Na2S x 9H2O solution under a N2 atmosphere, filter-sterilize and store 8% NaHCO3 solution under a N2-CO2 (80:20, v/v) gas mixture. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
HCl
(7.7 M, 25%) |
10.0 | ml | 2.5 | 68.568 |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 |
Distilled water | 990.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
ZnCl2 | 70 | mg | 0.07 | 0.514 |
MnCl2 x 4 H2O | 100 | mg | 0.1 | 0.505 |
H3BO3 | 6 | mg | 0.006 | 0.097 |
CoCl2 x 6 H2O | 190 | mg | 0.19 | 1.463 |
CuCl2 x 2 H2O | 2 | mg | 0.002 | 0.012 |
NiCl2 x 6 H2O | 24 | mg | 0.024 | 0.185 |
Na2MoO4 x 2 H2O | 36 | mg | 0.036 | 0.149 |
Distilled water | 1000 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 29.10.21 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | n.d. |
Equipment needed: | Anaerobic workbench, Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 4.869 | 83.309 |
MgCl2 x 6 H2O | 3.895 | 19.158 |
Casitone | 1.947 | - |
KCl | 0.331 | 4.441 |
NH4Cl | 0.243 | 4.551 |
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