Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NH4Cl | 1.0 | g | 1.109 | 20.726 | |
KH2PO4 | 0.3 | g | 0.333 | 2.444 | |
K2HPO4 | 0.3 | g | 0.333 | 1.91 | |
NaCl | 100.0 | g | 110.865 | 1897.07 | |
KCl | 4.0 | g | 4.435 | 59.485 | |
Na2SO4 | 3.0 | g | 3.326 | 23.416 | |
Sodium acetate | 1.0 | g | 1.109 | 13.515 | |
Sodium lactate | 1.0 | g | 1.109 | 9.893 | |
Yeast extract | 1.0 | g | 1.109 | - | |
Trypticase peptone
(BD-BBL) |
1.0 | g | 1.109 | - | |
FeCl2 solution | 1.0 | ml | - | - | |
Trace element solution | 1.0 | ml | - | - | |
Na2SeO3 x 5 H2O | 3.0 | �g | - | - | |
Resazurin | 1.0 | mg | 0.001 | 0.005 | |
Distilled water | 900.0 | ml | - | - | |
1 Mix components thoroughly and adjust pH to 7.0. Autoclave under a N2 atmosphere. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
MgCl2 x 6 H2O | 20.0 | g | 181.818 | 894.321 | |
CaCl2 x 2 H2O | 2.7 | g | 24.546 | 166.959 | |
Distilled water | 100.0 | ml | - | - | |
1 To complete the medium, combine Solutions A and B under a N2 atmosphere. Then, add per liter the following solution from an anaerobic stock: | |||||
Na2S x 9 H2O
(3%) |
10.0 | ml | - | - | |
2 Autoclave under a N2 atmosphere. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
HCl
(7.7 M, 25%) |
10.0 | ml | 2.5 | 68.568 |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 |
Distilled water | 990.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
ZnCl2 | 70 | mg | 0.07 | 0.514 |
MnCl2 x 4 H2O | 100 | mg | 0.1 | 0.505 |
H3BO3 | 6 | mg | 0.006 | 0.097 |
CoCl2 x 6 H2O | 190 | mg | 0.19 | 1.463 |
CuCl2 x 2 H2O | 2 | mg | 0.002 | 0.012 |
NiCl2 x 6 H2O | 24 | mg | 0.024 | 0.185 |
Na2MoO4 x 2 H2O | 36 | mg | 0.036 | 0.149 |
Distilled water | 1000 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.0 |
Equipment needed: | Anaerobic workbench, Autoclave |
Edit in Medium builder |
All strains for this medium: | 1 |