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J825. KOKO MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(BD-Difco)
1.00 g 0.979 -
2 1.00 g 0.979 -
3 1.00 g 0.979 -
4 1.60 g 1.567 8.997
5 1.00 g 0.979 -
6 0.50 g 0.49 9.155
7 0.16 g 0.157 0.636
8 0.06 g 0.059 0.4
9 SL-4 trace element solution 10.00 ml - -
10 Trace vitamins 10.00 ml - -
11 0.50 mg 4.9e-4 0.002
12 930.00 ml 909.239 50470.4
13
(10%)
50.00 ml - -
14
(*, 8%)
1.25 ml 0.211 2.517
15
(3%)
10.00 ml - -
16
(3%)
10.00 ml - -
Instructions:
  1. Mix components thoroughly and adjust pH to 7.0. Autoclave the medium under a N2 atmosphere. Add the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized): #13-16
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 8 other media (first defined in medium J340 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 1.723
2 0.2 g 0.2 0.719
3 SL-6 trace element solution 100.0 ml - -
4 900.0 ml 898.386 49868

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 928.793 51555.8
K2HPO4 1.567 8.997
NaH2PO4 x 2H2O 0.979 -
Tryptone 0.979 -
Peptone 0.979 -
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