Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
(NH4)2S4 | 1.300 | g | 1.297 | - | |
KH2PO4 | 1.500 | g | 1.497 | 11 | |
K2HPO4 | 2.000 | g | 1.996 | 11.46 | |
MgCl2 x 6 H2O | 0.500 | g | 0.499 | 2.454 | |
CaCl2 x 2 H2O | 0.075 | g | 0.075 | 0.509 | |
FeSO4 x 7 H2O | 1.250 | mg | 0.001 | 0.004 | |
FeCl2 solution | 1.000 | ml | - | - | |
Trace element solution | 1.000 | ml | - | - | |
Yeast extract | 2.000 | g | 1.996 | - | |
L-Cysteine HCl x H2O | 0.500 | g | 0.499 | - | |
Resazurin | 1.000 | g | 0.998 | 4.354 | |
Distilled water | 950.000 | ml | - | - | |
1 Mix components thoroughly and adjust pH to 6.0 with HCl. Autoclave under a N2 atmosphere. After cooling, aseptically and anaerobically add the following solution (filter-sterilized and stored under a N2 atmosphere): | |||||
Glucose
(10%) |
50.000 | ml | - | - | |
2 Finally, adjust pH to 7.0 with sterile anoxic 5% Na2CO2 solution (usually 30 - 40 ml to 1.0 L medium). |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
HCl
(7.7 M, 25%) |
10.0 | ml | 2.5 | 68.568 |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 |
Distilled water | 990.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
ZnCl2 | 70 | mg | 0.07 | 0.514 |
MnCl2 x 4 H2O | 100 | mg | 0.1 | 0.505 |
H3BO3 | 6 | mg | 0.006 | 0.097 |
CoCl2 x 6 H2O | 190 | mg | 0.19 | 1.463 |
CuCl2 x 2 H2O | 2 | mg | 0.002 | 0.012 |
NiCl2 x 6 H2O | 24 | mg | 0.024 | 0.185 |
Na2MoO4 x 2 H2O | 36 | mg | 0.036 | 0.149 |
Distilled water | 1000 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.0 |
Equipment needed: | Anaerobic workbench, Autoclave, Filter |
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Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
K2HPO4 | 1.996 | 11.46 |
Yeast extract | 1.996 | - |
KH2PO4 | 1.497 | 11 |
(NH4)2S4 | 1.297 | - |
Resazurin | 0.998 | 4.354 |
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