J826
826 .
MODIFIED CM3 MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J826 #
ml
Equipment needed: Anaerobic workbench
Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2S4 | 1.300 | g | 1.297 | - | |
| KH2PO4 | 1.500 | g | 1.497 | 11 | |
| K2HPO4 | 2.000 | g | 1.996 | 11.46 | |
| MgCl2 x 6 H2O | 0.500 | g | 0.499 | 2.454 | |
| CaCl2 x 2 H2O | 0.075 | g | 0.075 | 0.509 | |
| FeSO4 x 7 H2O | 1.250 | mg | 0.001 | 0.004 | |
| FeCl2 solution | 1.000 | ml | - | - | |
| Trace element solution | 1.000 | ml | - | - | |
| Yeast extract | 2.000 | g | 1.996 | - | |
| L-Cysteine HCl x H2O | 0.500 | g | 0.499 | - | |
| Resazurin | 1.000 | g | 0.998 | 4.354 | |
| Distilled water | 950.000 | ml | - | - | |
| 1 Mix components thoroughly and adjust pH to 6.0 with HCl. Autoclave under a N2 atmosphere. After cooling, aseptically and anaerobically add the following solution (filter-sterilized and stored under a N2 atmosphere): | |||||
|
Glucose
(10%) |
50.000 | ml | - | - | |
| 2 Finally, adjust pH to 7.0 with sterile anoxic 5% Na2CO2 solution (usually 30 - 40 ml to 1.0 L medium). | |||||
Solution occurs in 87
other media (first defined in Medium J187).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
|
HCl
(7.7 M, 25%) |
10.0 | ml | 2.5 | 68.568 |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 |
| Distilled water | 990.0 | ml | - | - |
Solution occurs in 89
other media (first defined in Medium J187).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| ZnCl2 | 70 | mg | 0.07 | 0.514 |
| MnCl2 x 4 H2O | 100 | mg | 0.1 | 0.505 |
| H3BO3 | 6 | mg | 0.006 | 0.097 |
| CoCl2 x 6 H2O | 190 | mg | 0.19 | 1.463 |
| CuCl2 x 2 H2O | 2 | mg | 0.002 | 0.012 |
| NiCl2 x 6 H2O | 24 | mg | 0.024 | 0.185 |
| Na2MoO4 x 2 H2O | 36 | mg | 0.036 | 0.149 |
| Distilled water | 1000 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter |
| Edit in Medium builder | |
Gas composition
- 2 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| K2HPO4 | 1.996 | 11.46 |
| Yeast extract | 1.996 | - |
| KH2PO4 | 1.497 | 11 |
| (NH4)2S4 | 1.297 | - |
| Resazurin | 0.998 | 4.354 |
| Show more | ||
