J830
830 .
DESULFOVIBRIO B0109P2 MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J830 #
ml
Equipment needed: Autoclave
Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 1.00 | g | 0.999 | 18.676 | |
| KH2PO4 | 0.30 | g | 0.3 | 2.202 | |
| K2HPO4 | 0.30 | g | 0.3 | 1.721 | |
| Na2SO4 | 2.80 | g | 2.797 | 19.693 | |
| NaCl | 20.00 | g | 19.98 | 341.889 | |
| KCl | 0.10 | g | 0.1 | 1.34 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| Na2S2O3 x 5 H2O | 3.16 | g | 3.157 | 12.72 | |
| Yeast extract | 0.10 | g | 0.1 | - | |
| Trace element solution | 1.00 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.5 | - | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| Distilled water | 900.00 | ml | - | - | |
| 1 To complete the medium, aseptically and anaerobically add the following stock solutions (per liter, final volume): | |||||
|
Sodium lactate
(1.0 M) |
20.00 | ml | - | - | |
|
MgCl2 x 6 H2O
(15%) |
20.00 | ml | - | - | |
|
NaHCO3
(5%) |
40.00 | ml | - | - | |
|
Na2S x 9 H2O
(2%) |
20.00 | ml | - | - | |
| 2 Mix ingredients and adjust pH to 7.4. Bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture tubes under the same gas mixture, seal with butyl rubber stoppers and autoclave. | |||||
| 3 Filter-sterilize NaHCO3 solution and store under a N2-CO2 (4:1, v/v) gas mixture. The other solutions are autoclaved and stored anaerobically under a N2 atmosphere. | |||||
| 4 Readjust pH of the medium to 7.4, if necessary. | |||||
Solution occurs in 38
other media (first defined in Medium J439).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
|
HCl
(25%) |
12.5 | ml | - | - |
| FeSO4 x 7 H2O | 2.1 | g | 2.1 | 7.554 |
| H3BO3 | 30.0 | mg | 0.03 | 0.485 |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | - |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 |
| ZnSO4 x 7 H2O | 144.0 | mg | 0.144 | 0.501 |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 |
| Distilled water | 987.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 7.4 |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter |
| Edit in Medium builder | |