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JCM 830 . DESULFOVIBRIO B0109P2 MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.00 g 0.999 18.676
KH2PO4 0.30 g 0.3 2.202
K2HPO4 0.30 g 0.3 1.721
Na2SO4 2.80 g 2.797 19.693
NaCl 20.00 g 19.98 341.889
KCl 0.10 g 0.1 1.34
CaCl2 x 2 H2O 0.10 g 0.1 0.68
Na2S2O3 x 5 H2O 3.16 g 3.157 12.72
Yeast extract 0.10 g 0.1 -
Trace element solution 1.00 ml - -
L-Cysteine HCl x H2O 0.50 g 0.5 -
Resazurin 1.00 mg 1.0e-3 0.004
Distilled water 900.00 ml - -
1 To complete the medium, aseptically and anaerobically add the following stock solutions (per liter, final volume):
Sodium lactate
(1.0 M)
20.00 ml - -
MgCl2 x 6 H2O
(15%)
20.00 ml - -
NaHCO3
(5%)
40.00 ml - -
Na2S x 9 H2O
(2%)
20.00 ml - -
2 Mix ingredients and adjust pH to 7.4. Bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture tubes under the same gas mixture, seal with butyl rubber stoppers and autoclave.
3 Filter-sterilize NaHCO3 solution and store under a N2-CO2 (4:1, v/v) gas mixture. The other solutions are autoclaved and stored anaerobically under a N2 atmosphere.
4 Readjust pH of the medium to 7.4, if necessary.
Solution occurs in 38 other media (first defined in Medium J439).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
12.5 ml - -
FeSO4 x 7 H2O 2.1 g 2.1 7.554
H3BO3 30.0 mg 0.03 0.485
MnCl2 x 4 H2O 100.0 mg 0.1 -
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
ZnSO4 x 7 H2O 144.0 mg 0.144 0.501
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 987.0 ml - -

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Complex medium
Final pH: 7.4
Equipment needed: Anaerobic workbench, Autoclave, Filter

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 19.98 341.889
Na2S2O3 x 5 H2O 3.157 12.72
Na2SO4 2.797 19.693
NH4Cl 0.999 18.676
L-Cysteine HCl x H2O 0.5 -
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