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JCM 833 . IP3-3 MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J833 #

ml
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 1.00 g 0.985 16.859
MgCl2 x 6 H2O 0.40 g 0.394 1.938
CaCl2 x 2 H2O 0.10 g 0.099 0.67
NH4Cl 0.25 g 0.246 4.605
KH2PO4 0.20 g 0.197 1.448
KCl 0.50 g 0.493 6.608
Sodium acetate x 3 H2O 6.80 g 6.7 -
L-Cysteine HCl x H2O 0.25 g 0.246 -
Resazurin 1.00 mg 9.9e-4 0.004
Distilled water 960.00 ml - -
1 Mix components and autoclave under a N2 atmosphere. After cooling, anaerobically add the following sterile solutions (autoclaved or *filter-sterilized) from anaerobic stocks:
NaHCO3
(*, 8%) 		30.00 ml - -
Trace element solution 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Vitamin solution (see Medium No. 403)
(*) 		1.00 ml - -
Thiamine solution (see Medium No. 403)
(*) 		1.00 ml - -
Vitamin B12 solution (see Medium No. 403)
(*) 		1.00 ml - -
Ampicillin
(*, 0.1 g/ml) 		10.00 ml - -
2 To complete the medium, dispense the medium into culture vessels leaving 40 to 50% volume of gas phase (e.g., 30 ml of the medium in a 50 ml serum bottle) and seal with butyl rubber stoppers. Replace the gas phase with a N2-CO2-H2 (8:1:1, v/v) gas mixture. Finally, add the following reducing solution and chlorosolvent (per liter):
Titanium citrate solution 10.00 ml - -
1,1,2-Trichloroethane 46.00 �l - -
3 Adjust pH to 7.0, if necessary.

Titanium citrate solution #

55
Equipment needed: Filter Centrifuge
Compound Amount Unit Conc. [g/L] Conc. [mM]
Titanium chloride 0.75 g 13.636 -
Trisodium citrate x 2 H2O 2.94 g 53.455 -
Distilled water 55.00 ml - -
1 Mix components and centrifuge at 8,000 x g for 10 min. Filter-sterilize the supernatant and store under a N2 atmosphere.

Trace element solution #

1000
Solution occurs in 38 other media (first defined in Medium J439).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		12.5 ml - -
FeSO4 x 7 H2O 2.1 g 2.1 7.554
H3BO3 30.0 mg 0.03 0.485
MnCl2 x 4 H2O 100.0 mg 0.1 -
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
ZnSO4 x 7 H2O 144.0 mg 0.144 0.501
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 987.0 ml - -

Selenite-tungstate solution #

1000
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Anaerobic workbench, Autoclave, Centrifuge, Filter
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sodium acetate x 3 H2O 6.7 -
NaCl 0.985 16.859
Trisodium citrate x 2 H2O 0.527 -
KCl 0.493 6.608
MgCl2 x 6 H2O 0.394 1.938
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