Name: SYFAC MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J837 #

Equipment needed: Anaerobic workbench Filter Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
1 Distribute the medium into culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of N₂-CO₂ (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Finally add per liter the following solution (autoclaved and stored under a N₂ atmosphere):
Sea Salt (Sigma)	35.0	g	34.757	-
Wolfe's mineral elixir	1.0	ml	-	-
Yeast extract (BD-Difco)	1.0	g	0.993	-
Resazurin	0.5	mg	5.0e-4	0.002
Distilled water	925.0	ml	-	-
2 Mix components thoroughly and autoclave under a N₂ atmosphere. Aseptically and anaerobically add the following solutions from sterile anaerobic stock solutions (autoclaved or *filter-sterilized):
Sodium acetate (1 M)	20.0	ml	-	-
Sodium fumarate (1 M)	20.0	ml	-	-
Trace vitamins (see Medium No. 197) (*)	10.0	ml	-	-
NaHCO₃ (*, 8%)	25.0	ml	-	-
Na₂S x 9 H₂O (5%)	6.0	ml	-	-

Wolfe's mineral elixir #

1000

Solution occurs in 8 other media (first defined in Medium J470).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
MgSO₄ x 7 H₂O	30.00	g	30	121.716
MnSO₄ x n H₂O	5.00	g	5	-
NaCl	10.00	g	10	171.116
FeSO₄ x 7 H₂O	1.00	g	1	3.597
CoCl₂ x 6 H₂O	1.80	g	1.8	13.863
CaCl₂ x 2 H₂O	1.00	g	1	6.802
ZnSO₄ x 7 H₂O	1.80	g	1.8	6.26
CuSO₄ x 5 H₂O	0.10	g	0.1	0.401
AlK(SO₄)₂ x 12 H₂O	0.18	g	0.18	-
H₃BO₃	0.10	g	0.1	1.617
Na₂MoO₄ x 2 H₂O	0.10	g	0.1	0.413
(NH₄)₂Ni(SO₄)₂ x 6 H₂O	2.80	g	2.8	7.089
Na₂WO₄ x 2 H₂O	0.10	g	0.1	0.303
Na₂SeO₄	0.10	g	0.1	0.529
Distilled water	1000.00	ml	-	-
1 First adjust pH to 1.0 with diluted H₂SO₄, then dissolve the salts.

Last modified:	29.10.21
Source:	JCM
Taxonomic range:	Bacteria
Medium type:	Complex medium
Final pH:	n.d.
Equipment needed:	Anaerobic workbench, Autoclave, Filter

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